MC4-R Antibody / Energy Homeostasis Receptor Antibody (RQ6059)

MC4-R Antibody A431 Cell IF. Immunofluorescent staining of FFPE human A431 cells using MC4-R antibody demonstrates prominent cytoplasmic and membranous staining (green), consistent with expression of Melanocortin Receptor 4 (MC4-R). MC4-R is a G protein-coupled receptor that mediates melanocortin-dependent signaling pathways involved in energy homeostasis, appetite regulation, neuroendocrine communication, and metabolic control. The observed staining pattern is consistent with the expected localization of a membrane-associated receptor and supports the utility of MC4-R antibody for studies of melanocortin receptor biology and hormone-mediated signaling pathways. DAPI nuclear stain (blue) identifies cell nuclei and provides contrast to the predominantly cytoplasmic and cell surface-associated distribution of MC4-R. HIER: steam section in pH6 citrate buffer for 20 min.

MC4-R Antibody Human Rectal Cancer IHC. Immunohistochemistry staining of FFPE human rectal cancer tissue using MC4-R antibody demonstrates moderate cytoplasmic and membranous staining within malignant gland-forming epithelial cells, consistent with expression of Melanocortin Receptor 4 (MC4-R). MC4-R is a G protein-coupled receptor that mediates melanocortin signaling pathways involved in energy homeostasis, neuroendocrine communication, and metabolic regulation. The observed staining pattern is consistent with the expected localization of a membrane-associated receptor and supports the utility of MC4-R antibody for studies of receptor-mediated signaling, endocrine regulation, and melanocortin pathway biology. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

MC4-R Antibody Human Mouse Rat Cell Line WB. Western blot analysis of human U-87 MG and HEK293 cell lysates, together with rat PC-12 and mouse Neuro-2a cell lysates, using MC4-R antibody demonstrates a distinct immunoreactive band at approximately 37-40 kDa across all samples, consistent with expression of Melanocortin Receptor 4 (MC4-R). MC4-R is a G protein-coupled receptor that mediates melanocortin-dependent signaling pathways involved in energy homeostasis, appetite regulation, neuroendocrine communication, and metabolic control. The observed cross-species detection pattern supports the conserved nature of MC4-R and demonstrates the utility of this antibody for studies of melanocortin receptor biology and hormone-mediated signaling pathways. Predicted molecular weight: ~37 kDa.

MC4-R Antibody U937 Flow Cytometry. Flow cytometric analysis of human U937 cells using MC4-R antibody demonstrates a clear rightward shift of the blue histogram (MC4-R antibody) relative to the green histogram (isotype control) and red histogram (cells alone), consistent with expression of Melanocortin Receptor 4 (MC4-R). MC4-R is a G protein-coupled receptor that mediates melanocortin signaling pathways involved in energy homeostasis, appetite regulation, neuroendocrine communication, and metabolic control. The observed staining pattern supports detection of endogenous MC4-R expression and demonstrates the utility of this antibody for studies of receptor-mediated signaling, endocrine regulation, and melanocortin pathway biology. Cells were stained with 1 ug antibody per million cells following blocking with goat sera.