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Home >> Antibodies >> MARCH2 Antibody / MARCHF2 / Membrane-associated RING finger protein 2

MARCH2 Antibody / MARCHF2 / Membrane-associated RING finger protein 2 (RQ8435)

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  Catalog No Formulation Size Price (USD)  
Image RQ8435 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 439
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Immunofluorescent staining of FFPE human HeLa cells with MARCH2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with MARCH2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) HEL, 2) PC-3 and 3) 293T cell lysate with MARCH2 antibody. Predicted molecular weight ~27 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9P0N8
Applications Western Blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
ELISA : 0.1-0.5ug/ml
Limitations This MARCH2 antibody is available for research use only.
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Description

MARCH2 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of TFRC and CD86, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. MARCH2 may be involved in endosomal trafficking through interaction with STX6.

Application Notes

Optimal dilution of the MARCH2 antibody should be determined by the researcher.

Immunogen

An E.coli-derived human recombinant protein (M1-V246) was used as the immunogen for the MARCH2 antibody.

Storage

After reconstitution, the MARCH2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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