MAD2B Antibody / REV7 Polyclonal Antibody (RQ8290)

MAD2B Antibody by IHC in Human Liver Cancer Immunohistochemistry of FFPE human liver cancer sections using MAD2B Antibody. This REV7 Polyclonal Antibody was tested following heat-induced epitope retrieval by boiling sections in pH 8 EDTA buffer for 20 minutes and allowing them to cool before staining.

MAD2B Antibody by IF in U-2 OS Cells Immunofluorescence staining of U-2 OS cells using MAD2B Antibody, shown in green, with DAPI nuclear counterstain in blue. The REV7 Polyclonal Antibody demonstrates prominent nuclear labeling together with detectable extranuclear signal, consistent with the reported nuclear and cytoplasmic localization of MAD2L2/REV7. Cells were prepared using heat-induced epitope retrieval in pH 6 citrate buffer.

MAD2B Antibody by WB in Multiple Human Cell Lines Western blot testing of MAD2B Antibody with A375 (lane 1), 293T (lane 2), HeLa (lane 3), K562 (lane 4), Jurkat (lane 5), THP-1 (lane 6) and SH-SY5Y (lane 7) cell lysates. The REV7 Polyclonal Antibody detects a clean, predominant band at approximately 24 kDa across all seven lysates, consistent with the predicted molecular weight of MAD2L2/REV7. Differences in band intensity likely reflect variation in target abundance among the tested cell lines.

MAD2B Antibody by FACS in HeLa Cells Flow cytometry testing of fixed and permeabilized HeLa cells using MAD2B Antibody at 1 ug per million cells. The REV7 Polyclonal Antibody produces a strong rightward fluorescence shift relative to both the isotype control and cells-alone control, demonstrating robust detection of endogenous MAD2L2/REV7. Cells were blocked with goat serum before testing.