Metadherin Antibody / MTDH Antibody (R31751)

Metadherin Antibody Human Endometrial Cancer IHC. Immunohistochemical staining of FFPE human endometrial cancer tissue using Metadherin antibody demonstrated strong cytoplasmic and membranous staining in malignant epithelial cells, consistent with the reported overexpression of MTDH in numerous solid tumors. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to staining, and the primary antibody was applied at 2 ug/ml. This Metadherin Antibody, also called an MTDH Antibody, is useful for investigating tumor progression, metastasis, oncogenic signaling, chemoresistance, and cancer biology.

Metadherin Antibody Human Stomach Cancer IHC. Immunohistochemical staining of FFPE human stomach cancer tissue using Metadherin antibody demonstrated predominantly cytoplasmic staining in malignant epithelial cells, consistent with the elevated expression of MTDH reported in gastric carcinoma and other aggressive solid tumors. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to staining, and the primary antibody was applied at 2 ug/ml. This Metadherin Antibody, also called an MTDH Antibody, is useful for investigating tumor progression, metastasis, oncogenic signaling, chemoresistance, and cancer biology.

Metadherin Antibody Human Lung Cancer Cell IF. Immunofluorescent staining of A549 cells using Metadherin antibody demonstrated predominantly cytoplasmic fluorescence with a fine punctate distribution, consistent with the reported intracellular localization of MTDH. Cells were treated with enzyme antigen retrieval reagent for 15 minutes, blocked with 10% goat serum, and incubated with the primary antibody (5 ug/ml) overnight at 4°C. Cy3-conjugated goat anti-rabbit IgG was used as the secondary antibody, and nuclei were counterstained with DAPI (blue). This Metadherin Antibody, also called an MTDH Antibody, is useful for investigating tumor progression, metastasis, oncogenic signaling, chemoresistance, and cancer biology.

Metadherin Antibody Mouse Brain IHC. Immunohistochemical staining of FFPE mouse brain tissue using Metadherin antibody demonstrated predominantly cytoplasmic staining in neurons, consistent with the physiological expression of MTDH in the central nervous system. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to staining, and the primary antibody was applied at 2 ug/ml. This Metadherin Antibody, also called an MTDH Antibody, is useful for investigating neurobiology, oncogenic signaling, inflammation, and cancer biology.

Metadherin Antibody Rat Brain IHC. Immunohistochemical staining of FFPE rat brain tissue using Metadherin antibody demonstrated predominantly cytoplasmic staining in neurons throughout the cerebral cortex and hippocampal region, consistent with the normal expression of MTDH in the central nervous system. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer prior to staining, and the primary antibody was applied at 2 ug/ml. This Metadherin Antibody, also called an MTDH Antibody, is useful for investigating neurobiology, oncogenic signaling, inflammation, and cancer biology.

Metadherin Antibody Human, Mouse and Rat WB. Western blot testing of human MCF-7 (lane 1), PC-3 (lane 2), A375 (lane 3), and HeLa (lane 4) cell lysates together with rat testis (lane 5) and mouse testis (lane 6) lysates using Metadherin antibody detected immunoreactive bands at approximately 70-75 kDa. Although the predicted molecular weight of MTDH is approximately 64 kDa, the protein is frequently observed as a higher-migrating doublet due to post-translational modifications, including phosphorylation and differential glycosylation. This Metadherin Antibody, also called an MTDH Antibody, is useful for investigating tumor progression, metastasis, oncogenic signaling, chemoresistance, and cancer biology.

Metadherin Antibody Human Breast Cancer Cell IP. Immunoprecipitation of MTDH from human MCF-7 cell lysate using Metadherin antibody followed by Western blot analysis demonstrated efficient enrichment of the characteristic 70-75 kDa MTDH doublet. Lane 1 shows the input lysate, lane 2 is the rabbit IgG negative control, and lane 3 contains the immunoprecipitated MTDH complex obtained using 2 ug of antibody from 500 ug of lysate. The observed doublet is consistent with the known post-translationally modified forms of MTDH, which commonly migrate above the predicted molecular weight of approximately 64 kDa. This Metadherin Antibody, also called an MTDH Antibody, is useful for investigating protein interactions, tumor progression, metastasis, oncogenic signaling, and cancer biology.

Metadherin Antibody Human Breast Cancer Cell FACS. Flow cytometry analysis of fixed and permeabilized human MCF-7 cells using Metadherin antibody demonstrated a clear rightward shift of the stained cell population (blue) relative to the isotype control (green), indicating specific intracellular detection of MTDH. Cells without primary and secondary antibodies served as the blank control (red), and cells were blocked with normal goat serum prior to staining. This Metadherin Antibody, also called an MTDH Antibody, is useful for investigating tumor progression, metastasis, oncogenic signaling, chemoresistance, and cancer biology.