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Home >> Antibodies >> Ly6c Antibody

Ly6c Antibody (RQ5893)

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  Catalog No Formulation Size Price (USD)  
Image RQ5893 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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IHC staining of FFPE rat spleen with Ly6c antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat spleen with Ly6c antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of mouse 1) RAW264.7 and 2) ANA-1 cell lysate with Ly6c antibody. Predicted molecular weight ~14 kDa.
Flow cytometry testing of mouse spleen cells with Ly6c antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ly6c antibody.
IHC staining of FFPE mouse spleen with Ly6c antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P0CW02
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry : 1-2ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Ly6c antibody is available for research use only.
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Description

Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells.

Application Notes

Optimal dilution of the Ly6c antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids L27-G109) was used as the immunogen for the Ly6c antibody.

Storage

After reconstitution, the Ly6c antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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