LFA-1 Antibody / CD18 Polyclonal Antibody (R34026)

LFA-1 Antibody Western Blot of K562 and U937 Cells. Western blot testing of human K562 (A) and U937 (B) cell lysates with LFA-1 Antibody at 0.3 ug/ml. Both lysates show a strong predominant band at approximately 85-95 kDa, consistent with the expected migration of glycosylated CD18/ITGB2. The similar target-sized signal in both samples demonstrates detection of integrin beta 2 by this CD18 Polyclonal Antibody.

LFA-1 Antibody Western Blot of U937, K562 and MOLT-4 Cells. Western blot testing of human U937 (A), K562 (B), and MOLT-4 (C) cell lysates with LFA-1 Antibody. A predominant band is detected at approximately 85-95 kDa in each lysate, consistent with glycosylated CD18/ITGB2. Signal intensity varies among the three samples, with particularly strong target-sized detection in U937 cells. The results demonstrate recognition of integrin beta 2 in multiple human hematopoietic cell lines by this CD18 Polyclonal Antibody.

LFA-1 Antibody in Human Spleen IHC. Immunohistochemistry staining of FFPE human spleen tissue with LFA-1 Antibody at 5 ug/ml. Heat-induced epitope retrieval was performed by steaming sections in pH 6 citrate buffer, followed by AP-based detection. Distinct red staining highlights scattered CD18-positive cells throughout the splenic lymphoid tissue, with minimal staining in many surrounding cells. This cellular distribution demonstrates detection of the ITGB2-encoded beta 2 integrin subunit by this CD18 Polyclonal Antibody.