CD18 Antibody / ITGB2 Polyclonal Antibody (R31562)

CD18 Antibody in Human Tonsil IF. Immunofluorescence staining of FFPE human tonsil tissue with CD18 Antibody at 5 ug/ml. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer, followed by blocking with 10% goat serum and overnight primary antibody incubation at 4oC. DyLight 488-conjugated goat anti-rabbit IgG was used for detection. Green CD18 signal is visible in discrete populations of cells throughout the tonsillar lymphoid tissue, with nuclei counterstained blue. This ITGB2 Polyclonal Antibody demonstrates heterogeneous CD18 expression among cells of human tonsil.

CD18 Antibody in Human Intestinal Cancer IHC. Immunohistochemistry staining of FFPE human intestinal cancer tissue with CD18 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer before antibody staining. Strong brown immunoreactivity is concentrated in a population of infiltrating cells within the tissue, while surrounding structures show substantially less staining. This ITGB2 Polyclonal Antibody demonstrates CD18 detection in immune-cell-rich regions of human intestinal cancer tissue.

CD18 Antibody in Human Lung Cancer IHC. Immunohistochemistry staining of FFPE human lung cancer tissue with CD18 Antibody at 2 ug/ml. Heat-induced epitope retrieval was performed in pH 8 EDTA buffer before antibody staining. The image shows an intensely CD18-positive cluster of infiltrating cells surrounded by more weakly stained tissue, producing strong contrast between the positive leukocyte population and adjacent structures. This staining supports tissue detection of integrin beta 2 with the ITGB2 Polyclonal Antibody.

CD18 Antibody Western Blot of Jurkat and HeLa Cells. Western blot testing of human Jurkat (lane 1) and HeLa (lane 2) whole cell lysates with CD18 Antibody at 0.5 ug/ml. A strong band is detected in Jurkat cells at approximately 85-90 kDa, consistent with glycosylated CD18/ITGB2, while the corresponding signal is essentially absent from HeLa cells. A prominent lower band near approximately 20 kDa is present in the HeLa lane and is inconsistent with full-length CD18. The differential target-sized signal demonstrates detection of ITGB2 in Jurkat cells by this ITGB2 Polyclonal Antibody.