LAMP1 Antibody Lysosomal Membrane Glycoprotein Antibody R30920

	Catalog No	Formulation	Size	Price (USD)
	R30920	0.5mg/ml if reconstituted with 0.2ml sterile DI water	100 ug	449
Formulation

LAMP1 Antibody Human, Mouse WB. Western blot analysis of human and mouse lysates using LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody. Lane 1: human HepG2 cells; Lane 2: human Jurkat cells; Lane 3: human HeLa cells; Lane 4: mouse brain; Lane 5: mouse lung. LAMP1 is detected as broad immunoreactive bands ranging from approximately 95 to 120 kDa, consistent with the extensive N-linked glycosylation of this lysosomal membrane glycoprotein, which frequently migrates well above its calculated molecular weight and may be observed at up to approximately 140 kDa. This LAMP1 Antibody is suitable for investigating lysosomal membrane biology, intracellular trafficking, autophagy, and lysosome-associated cellular processes across multiple species.

LAMP1 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody following heat-induced epitope retrieval in pH8 EDTA buffer. The antibody was applied at 2 ug/ml and detected using an HRP-conjugated secondary antibody with DAB chromogen. Strong granular cytoplasmic staining is observed throughout the malignant hepatocytes, consistent with abundant lysosomal localization, while the surrounding stromal tissue exhibits comparatively weaker immunoreactivity. This LAMP1 Antibody is suitable for investigating lysosomal membrane biology, intracellular trafficking, autophagy, and liver cancer progression.

LAMP1 Antibody Human Epithelial Cell WB. Western blot analysis of human cell lysates using LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody. Lane 1: human Caco-2 cells; Lane 2: human HeLa cells; Lane 3: human U2OS cells. A broad immunoreactive band is detected at approximately 95-120 kDa in all three cell lines. Although the predicted molecular weight of the LAMP1 polypeptide is approximately 45 kDa, extensive N-linked glycosylation substantially increases its apparent molecular weight, and LAMP1 is routinely observed between approximately 95 and 120 kDa. This LAMP1 Antibody is suitable for investigating lysosomal membrane biology, intracellular trafficking, autophagy, and lysosome-associated cellular processes.

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LAMP1 Antibody Human Tonsil IHC. Immunohistochemical staining of FFPE human tonsil tissue using LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody following heat-induced epitope retrieval in pH8 EDTA buffer. The antibody was applied at 2 ug/ml and detected using an HRP-conjugated secondary antibody with DAB chromogen. Distinct granular cytoplasmic staining is observed in scattered immune cells throughout the lymphoid tissue, consistent with lysosomal localization, while the surrounding lymphocyte population exhibits comparatively weaker staining. This LAMP1 Antibody is suitable for investigating lysosomal membrane biology, intracellular trafficking, autophagy, and immune cell function.

LAMP1 Antibody Human Liver Cancer IHC. Immunohistochemical staining of FFPE human liver cancer tissue using LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody following heat-induced epitope retrieval in pH8 EDTA buffer. The antibody was applied at 2 ug/ml and detected using an HRP-conjugated secondary antibody with DAB chromogen. Strong granular cytoplasmic staining is observed throughout the malignant hepatocytes, consistent with abundant lysosomal localization, while the surrounding stromal tissue exhibits comparatively weaker immunoreactivity. This LAMP1 Antibody is suitable for investigating lysosomal membrane biology, intracellular trafficking, autophagy, and liver cancer progression.

LAMP1 Antibody Human Epithelial Cell WB. Western blot analysis of human cell lysates using LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody. Lane 1: human Caco-2 cells; Lane 2: human HeLa cells; Lane 3: human U2OS cells. A broad immunoreactive band is detected at approximately 95-120 kDa in all three cell lines. Although the predicted molecular weight of the LAMP1 polypeptide is approximately 45 kDa, extensive N-linked glycosylation substantially increases its apparent molecular weight, and LAMP1 is routinely observed between approximately 95 and 120 kDa. This LAMP1 Antibody is suitable for investigating lysosomal membrane biology, intracellular trafficking, autophagy, and lysosome-associated cellular processes.

Availability	1-3 business days
Species Reactivity	Human
Format	Antigen affinity purified
Host	Rabbit
Clonality	Polyclonal (rabbit origin)
Isotype	Rabbit IgG
Purity	Antigen affinity
Buffer	Lyophilized from 1X PBS with 2% Trehalose
UniProt	P11279
Applications	Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml
Limitations	This LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody is available for research use only.
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Description

LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody recognizes lysosomal associated membrane protein 1 (LAMP1), a highly glycosylated type I transmembrane protein that represents one of the most abundant structural components of the lysosomal membrane. LAMP1 is broadly expressed in mammalian cells and plays an essential role in maintaining lysosomal integrity, protecting the limiting membrane from degradation by luminal hydrolases, and supporting intracellular trafficking. Although predominantly localized to lysosomes and late endosomes, LAMP1 can also be transiently expressed at the plasma membrane during lysosomal exocytosis. A LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody provides a reliable tool for studying lysosome organization, membrane dynamics, and intracellular protein trafficking across numerous biological systems.

LAMP1 participates in multiple cellular processes including endosomal maturation, autophagy, lysosome biogenesis, membrane fusion, and degradation of intracellular cargo. Together with LAMP2, it contributes to the structural stability of lysosomes while facilitating vesicular transport and communication between endocytic and autophagic pathways. Surface expression of LAMP1 increases during lysosomal exocytosis, reflecting activation of secretory lysosomes in cytotoxic T lymphocytes, natural killer cells, macrophages, and other immune cells. Consequently, LAMP1 has become an important marker for lysosomal function, intracellular vesicle trafficking, and regulated secretion in both basic and translational research.

Altered LAMP1 expression or localization has been associated with cancer, lysosomal storage disorders, neurodegenerative disease, infectious disease, immune dysfunction, and metabolic disorders. Increased lysosomal activity and LAMP1 expression have been reported in numerous malignancies, where they may contribute to tumor cell survival, invasion, metastasis, therapeutic resistance, and remodeling of the tumor microenvironment. Changes in lysosomal membrane composition have also been linked to defective autophagy, impaired protein turnover, inflammatory responses, and cellular adaptation to metabolic stress. A LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody supports investigations into these diverse biological processes while enabling visualization of lysosomal abundance, distribution, and membrane integrity.

LAMP1 antibodies are suitable for applications including Western blotting, immunohistochemistry, immunofluorescence, flow cytometry, immunoprecipitation, and related protein detection methods when validated for the specific application. Because LAMP1 is extensively glycosylated, the apparent molecular weight frequently exceeds the calculated molecular mass and may vary among tissues and cell types depending on glycosylation status. NSJ Bioreagents provides carefully validated antibodies to support investigations of lysosome biology, intracellular trafficking, autophagy, immune regulation, and cancer research. A LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody is a valuable research reagent for detecting LAMP1 and advancing studies of lysosomal membrane organization, vesicular transport, autophagy, and disease-associated cellular remodeling.

This LAMP1 / CD107A antibody is part of a broader CD antibody panel offered by NSJ Bioreagents.

Application Notes

The stated application concentrations are suggested starting points. Titration of the LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

Amino acids 403-417 (YLVGRKRSHAGYQTI) were used as the immunogen for this LAMP1 antibody.

Storage

After reconstitution, the LAMP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

LAMP1 antibody, Lysosomal Membrane Glycoprotein antibody, Lysosomal Associated Membrane Protein 1 antibody, Lysosome Associated Membrane Glycoprotein 1 antibody, CD107a antibody, Lysosome Marker antibody

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LAMP1 Antibody / Lysosomal Membrane Glycoprotein Antibody (R30920)

Description

Application Notes

Immunogen

Storage

Alternate Names

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Area of Research

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Protocols

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NSJ Bioreagents