KRT14 Antibody KRT14/4128 / Cytokeratin 14 Monoclonal Antibody [clone KRT14/4128] (V4032)

KRT14 Antibody KRT14/4128 by WB in Human A431 Cells. Western blot analysis of human A431 epidermoid carcinoma cell lysate using KRT14 Antibody KRT14/4128. A prominent immunoreactive band is observed at approximately 53 kDa, consistent with the predicted molecular weight of cytokeratin 14. The band is relatively broad, with the strongest signal concentrated near the expected molecular weight. The Cytokeratin 14 Monoclonal Antibody demonstrates recognition of a protein migrating at the expected size of KRT14 in A431 cell lysate.

KRT14 Antibody KRT14/4128 by IHC in Human Prostate. Immunohistochemistry of FFPE human prostate tissue using KRT14 Antibody KRT14/4128. Brown cytoplasmic staining is concentrated in the basal epithelial cells surrounding prostatic glands, while the luminal epithelial compartments exhibit comparatively weak or absent staining. The Cytokeratin 14 Monoclonal Antibody highlights the basal cell distribution of KRT14, consistent with its expression in basal epithelial populations. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM Tris with 1 mM EDTA, pH 9, for 20 minutes, followed by cooling before staining.

KRT14 Antibody KRT14/4128 by Protein Microarray Analysis. HuProt protein microarray analysis of KRT14 Antibody KRT14/4128 against more than 19,000 full-length human proteins. The graph displays a dominant binding signal for KRT14, separated from the remaining proteins represented on the array. The Cytokeratin 14 Monoclonal Antibody demonstrates preferential recognition of its intended target under the tested microarray conditions. The Z-score represents the strength of the antibody-binding signal relative to the array background, while the S-score reflects the separation between the intended target and the next-highest ranked protein.

KRT14 Antibody KRT14/4128 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free KRT14 Antibody KRT14/4128 under reducing (R) and non-reducing (NR) conditions, with 2 ug of antibody loaded per lane. The non-reducing lane shows a prominent band migrating near 150 kDa, consistent with intact immunoglobulin. Under reducing conditions, bands are observed near 50 kDa and 25 kDa, corresponding to the immunoglobulin heavy and light chains, respectively. The Cytokeratin 14 Monoclonal Antibody demonstrates the expected electrophoretic profile of a purified mouse monoclonal antibody preparation.