- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Klotho Antibody (Center Region) (F54137)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
F54137-0.2ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.2 ml | 439 | |
|
|
F54137-0.05ML | In 1X PBS, pH 7.4, with 0.09% sodium azide | 0.05 ml | 205 |
|
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity |
| UniProt | Q9UEF7 |
| Applications | Western Blot : 1:2000 |
| Limitations | This Klotho antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
The KL gene encodes Klotho, a membrane-associated protein with important functions in endocrine regulation and mineral homeostasis. Klotho is particularly abundant in renal tissue and contains a large extracellular region with two related domains known as KL1 and KL2. Its biological activities connect kidney function with systemic regulation of phosphate and vitamin D metabolism.
Membrane-associated Klotho forms receptor complexes with fibroblast growth factor receptors and is required for efficient signaling by the endocrine hormone FGF23. This interaction alters receptor ligand-binding properties and permits FGF23-dependent signaling in Klotho-expressing cells. In renal tissue, activation of this pathway reduces phosphate reabsorption and influences enzymes controlling the production and degradation of active vitamin D.
In addition to the membrane-associated protein, extracellular portions of Klotho can be released through proteolytic cleavage. Soluble Klotho has been studied in physiological processes involving ion transport, cellular stress responses and tissue maintenance. The relationship between altered Klotho expression and age-associated changes has also generated substantial interest in renal, cardiovascular and neuroscience research.
This rabbit polyclonal antibody was generated against a synthetic peptide from within amino acids 402-436 of human Klotho. NSJ Bioreagents provides this reagent for studies of KL protein expression and the molecular pathways connecting Klotho with renal physiology, endocrine signaling and mineral homeostasis.
For additional information on Klotho biology and tissue-based validation, visit our Klotho Antibody / KL Antibody page.
The stated application concentrations are suggested starting points. Titration of the Klotho antibody may be required due to differences in protocols and secondary/substrate sensitivity.
A portion of amino acids 402-436 from human Klotho was used as the immunogen for the Klotho antibody.
Aliquot the Klotho antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
![Western blot testing of human prostate lysate with GPX7 antibody at 0.3ug/ml with [B] and without [A] blocking/immunizing peptide. The expected ~20 kDa band and the additional ~40 kDa band are both blocked by the immunizing peptide.](https://www.nsjbio.com/plp/thumb_image/img-R33292-100UG-1.jpg?v=1788648665 "Western blot testing of human prostate lysate with GPX7 antibody at 0.3ug/ml with [B] and without [A] blocking/immunizing peptide. The expected ~20 kDa band and the additional ~40 kDa band are both blocked by the immunizing peptide.")
![Western blot testing of human [A], mouse [B] and rat [C] lung lysates with DLL4 antibody at 0.3ug/ml. Predicted/observed molecular weight 75~85kDa.](https://www.nsjbio.com/plp/thumb_image/img-R34555-100UG-1.jpg?v=1788648665 "Western blot testing of human [A], mouse [B] and rat [C] lung lysates with DLL4 antibody at 0.3ug/ml. Predicted/observed molecular weight 75~85kDa.")
![HEK293 lysate overexpressing human HOXA1-MYC probed with HOXA1 [1ug/ml] in Lane A and anti-MYC tag [1/1000] in lane C. Mock-transfected HEK293 probed with HOXA1 antibody [1ug/ml] in Lane B. Predicted molecular weight: ~37/15kDa (isoforms a/b).](https://www.nsjbio.com/plp/thumb_image/img-R35385-100UG-1.jpg?v=1788648665 "HEK293 lysate overexpressing human HOXA1-MYC probed with HOXA1 [1ug/ml] in Lane A and anti-MYC tag [1/1000] in lane C. Mock-transfected HEK293 probed with HOXA1 antibody [1ug/ml] in Lane B. Predicted molecular weight: ~37/15kDa (isoforms a/b).")
