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Home >> Antibodies >> KLF4 Antibody

KLF4 Antibody (F48148)

  Catalog No Formulation Size Price (USD)  
Image F48148-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 329
Image
F48148-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 135
Fluorescent confocal image of SY5Y cells stained with KLF4 antibody at 1:100. Note the highly specific localization of immunoreactivity to the nuclei.
Immunofluorescent analysis of U251 cells using KLF4 antibody; Ab was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG was used as the secondary Ab (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
Fluorescent confocal image of HeLa cells stained with KLF4 antibody at 1:100. Note the highly specific localization of the immunoreactivity to the nuclei but not the cytoplasm.
Western blot analysis of KLF4 antibody and MDA-MB231, NCI-H460 lysate. Predicted molecular weight: 50-60 kDa + possible ~75 kDa (phosphorylated form).
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt O43474
Applications Western blot : 1:1000
Immunofluorescence : 1:10-1:50
Limitations This KLF4 antibody is available for research use only.
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Description

Kruppel-like factor 4 (KLF4) is a transcription factor involved in both proliferation and differentiation in the colon.

Application Notes

Titration of the KLF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 481-504 from the human protein was used as the immunogen for this KLF4 antibody.

Storage

Aliquot the KLF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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