KLF4 Antibody for IF (F48148)

KLF4 Antibody SH-SY5Y Cell IF. Immunofluorescent staining of fixed and permeabilized human SH-SY5Y neuroblastoma cells using KLF4 Antibody for IF at a 1:100 dilution. KLF4 immunoreactivity (green) is highly enriched within the nuclei, while the actin cytoskeleton is labeled in red and nuclei are counterstained with DAPI (blue). The staining pattern is consistent with the expected nuclear localization of this transcription factor. This KLF4 Antibody for IF is suitable for studies of nuclear protein localization, transcriptional regulation, stem cell biology, and neuronal cell research.

KLF4 Antibody U251 Cell IF. Immunofluorescent staining of fixed and permeabilized human U251 glioma cells using KLF4 Antibody for IF at a 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody (green), while F-actin was counterstained with DyLight Fluor 554-conjugated phalloidin (red). KLF4 staining is predominantly localized to the nuclei, consistent with its function as a transcription factor. This KLF4 Antibody for IF is suitable for studies of nuclear localization, transcriptional regulation, stem cell biology, and cancer cell signaling.

KLF4 Antibody HeLa Cell IF. Immunofluorescent staining of fixed and permeabilized human HeLa cells using KLF4 Antibody for IF at a 1:100 dilution. KLF4 immunoreactivity (green) is specifically localized to the nuclei with minimal cytoplasmic staining, consistent with the expected localization of this transcription factor. The actin cytoskeleton is shown in red, highlighting the clear nuclear distribution of the target protein. This KLF4 Antibody for IF is suitable for studies of nuclear protein localization, transcriptional regulation, stem cell biology, and cancer cell research.

Western blot analysis of KLF4 antibody and MDA-MB231, NCI-H460 lysate. Predicted molecular weight: 50-60 kDa + possible ~75 kDa (phosphorylated form).