Ki-67 Antibody Western Blot / MKI67 Western Blot Antibody [clone MKI67/2463] (V7435)

IHC testing of FFPE human tonsil stained with Ki67 antibody (MKI67/2463). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human skin stained with Ki67 antibody (MKI67/2463). Required HIER: boiling tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.

FACS testing of human HeLa cells with Ki67 antibody (blue, clone MKI67/2463) and isotype control (red). Cells were trypsinized and 2-4% PFA-fixed prior to staining.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Ki67 antibody (clone MKI67/2463). These results demonstrate the foremost specificity of the MKI67/2463 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Western blot testing of human Raji and Ramos cell lysate with Ki-67 Antibody Western Blot (clone MKI67/2463). Expected molecular weight ~350 kDa.

SDS-PAGE analysis of purified, BSA-free Ki-67 Antibody for Western Blot (clone MKI67/2463) as confirmation of integrity and purity.