KHSRP Antibody 4F10D2 / KH-Type Splicing Regulatory Protein Antibody [clone 4F10D2] (RQ7124)

KHSRP Antibody 4F10D2 Immunofluorescence in MCF7 Cells. Immunofluorescence staining of FFPE human MCF7 cells was performed with KHSRP Antibody 4F10D2 (green) and DAPI nuclear stain (blue) following heat-induced epitope retrieval by steaming in pH 6 citrate buffer for 20 min. Strong KHSRP fluorescence is concentrated within the nuclei, with heterogeneous intranuclear staining visible among the cells. The localization supports detection with a KH-Type Splicing Regulatory Protein Antibody by immunofluorescence.

KHSRP Antibody 4F10D2 Western Blot in HeLa and MCF7 Cells. Western blot testing of human HeLa (lane 1) and MCF7 (lane 2) cell lysates was performed with KHSRP Antibody 4F10D2. A strong predominant band is detected near the predicted molecular weight of approximately 73 kDa in both samples, with relatively little additional background signal. The result demonstrates recognition of KH-type splicing regulatory protein in human cell lysates.

KHSRP Antibody 4F10D2 Western Blot in Human, Rat and Mouse Samples. Western blot testing was performed with KHSRP Antibody 4F10D2 using human HeLa (lane 1), MCF7 (lane 2), 293T (lane 3), and Jurkat (lane 4) cell lysates, rat brain (lane 5), mouse brain (lane 6), and mouse stomach (lane 7) tissue lysates. A prominent band is detected near the expected ~73 kDa region across the samples, with particularly strong signal in the brain lysates. This broad detection supports recognition by the KH-Type Splicing Regulatory Protein Antibody across multiple sample types.