Keratin 7 Antibody Cocktail KRT7/760 + KRT7/903 / Mouse Monoclonals [clone KRT7/760 + KRT7/903] (V2659)

Description

Keratin 7 Antibody Cocktail KRT7/760 + KRT7/903 recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

For an alternative antibody targeting the same protein, see our Cytokeratin 7 Antibody OV-TL12/30 (V2654), a mouse monoclonal antibody validated for WB, IHC, IF, and flow cytometry.

Application Notes

Optimal dilution of the Keratin 7 Antibody Cocktail KRT7/760 + KRT7/903 should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant full-length human KRT7 protein was used as the immunogen for the Keratin 7 Antibody Cocktail KRT7/760 + KRT7/903.

Storage

Store the Keratin 7 Antibody Cocktail KRT7/760 + KRT7/903 at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Alternate Names

Keratin 7 antibody, CK7 antibody, CK-7 antibody, KRT7 antibody, Cytokeratin-7 antibody, Keratin Type II Cytoskeletal 7 antibody, Type II Cytokeratin 7 antibody, Cytokeratin 7/CK7 antibody, K7 antibody