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Home >> Antibodies >> ISL1 Antibody / Islet 1

ISL1 Antibody / Islet 1 (R32744)

  Catalog No Formulation Size Price (USD)  
Image R32744 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of 1) rat PC12 and 2) human MCF7 lysate with ISL1 antibody at 0.5ug/ml. Predicted molecular weight ~39 kDa.
IHC testing of FFPE human breast cancer tissue with ISL1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Availability 1-3 business days
Species Reactivity Human, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P61371
Localization Cytoplasmic, nuclear
Applications Western Blot : 0.5-1ug/ml
IHC (FFPE) : 1-2ug/ml
Limitations This ISL1 antibody is available for research use only.
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Description

Insulin gene enhancer protein ISL-1 is a protein that in humans is encoded by the isl1 gene. This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Application Notes

Optimal dilution of the ISL1 antibody should be determined by the researcher.

Immunogen

Amino acids 118-161 (DEFALREDGLFCRADHDVVERASLGAGDPLSPLHPARPLQMAAE) from the human protein were used as the immunogen for the ISL1 antibody.

Storage

After reconstitution, the ISL1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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