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Home >> Antibodies >> Mouse ISG15 Antibody / ISG15 Polyclonal Antibody

Mouse ISG15 Antibody / ISG15 Polyclonal Antibody (R32374)

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Image R32374 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Mouse ISG15 Antibody by WB in Mouse Spleen. Western blot analysis of mouse spleen tissue lysate using ISG15 Polyclonal Antibody. A band is detected at approximately 17 kDa, consistent with the expected molecular weight of unconjugated ISG15.
Mouse ISG15 Antibody by IHC in Mouse Kidney. Immunohistochemical staining of FFPE mouse kidney tissue using ISG15 Polyclonal Antibody. Brown immunoreactivity is observed in scattered renal tubular structures, with blue hematoxylin nuclear counterstaining. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6.0, for 20 minutes.
Mouse ISG15 Antibody by WB in Mouse Tissues. Western blot analysis of mouse tissue lysates using ISG15 Polyclonal Antibody. Lane 1: spleen; Lane 2: heart; Lane 3: lung; Lane 4: liver; Lane 5: kidney. Bands are detected at approximately 15-17 kDa across all five tissues, consistent with the expected molecular weight of unconjugated ISG15.
Species Reactivity Mouse
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt Q64339
Localization Cytoplasmic
Applications Western Blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Limitations This Mouse ISG15 Antibody / ISG15 Polyclonal Antibody is available for research use only.
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Description

Mouse ISG15 Antibody / ISG15 Polyclonal Antibody recognizes interferon-stimulated gene 15 protein, an inducible ubiquitin-like modifier involved in innate immunity and cellular defense mechanisms. Encoded by the Isg15 gene, this protein is expressed in response to interferon signaling and participates in the regulation of antiviral responses, inflammation, and protein modification. ISG15 contains two ubiquitin-like domains and undergoes C-terminal processing to expose a diglycine motif required for conjugation to intracellular substrates. The mature protein can exist in an unconjugated form or become covalently attached to other proteins through ISGylation.

ISGylation is mediated by a coordinated enzymatic system involving the E1 activating enzyme UBA7, the E2 conjugating enzyme UBE2L6, and E3 ligases that promote substrate modification. In mice, the E3 ligase HERC6 has an important role in ISG15 conjugation, distinguishing aspects of the murine pathway from the human system, where HERC5 is prominent. ISGylation can modify proteins involved in translation, metabolism, signaling, and host defense, although its effects vary according to the substrate and experimental context.

Mouse ISG15 has been extensively investigated in models of viral infection. Interferon-dependent induction of Isg15 contributes to protective responses against certain pathogens, and genetic studies have helped clarify its role in restricting viral replication. Some viruses produce proteins that interfere with ISG15 conjugation or remove ISG15 from modified substrates. These interactions demonstrate how pathogens can target host ubiquitin-like modification systems to influence infection outcomes.

ISG15 biology also intersects with regulation of interferon signaling. The protease USP18 removes ISG15 from conjugated proteins and independently functions as a negative regulator of type I interferon receptor signaling. However, the relationship between ISG15 and USP18 differs between mice and humans. Human ISG15 stabilizes USP18 and contributes to interferon regulation through this interaction, whereas the corresponding mechanism is less prominent in mice. This species-dependent distinction is important when comparing inflammatory phenotypes and interpreting experimental findings.

Expression of ISG15 has additionally been investigated in inflammatory tissues, immune cell populations, and models of tissue injury. Its induction reflects activation of interferon-responsive pathways, but the presence of ISG15 does not independently establish a particular antiviral or inflammatory mechanism. NSJ Bioreagents offers this Mouse ISG15 Antibody to support investigations of murine interferon responses, ISGylation, antiviral immunity, and the regulation of ubiquitin-like protein modification during cellular stress.

For additional research into interferon-stimulated gene 15, explore our ISG15 Antibody, which provides complementary validation data for investigating ISG15 expression and interferon-responsive signaling.

Application Notes

Optimal dilution of the Mouse ISG15 Antibody / ISG15 Polyclonal Antibody should be determined by the researcher.

Immunogen

Amino acids A2-G155 from the mouse protein were used as the immunogen for the Mouse ISG15 Antibody.

Storage

After reconstitution, the Mouse ISG15 Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

Mouse interferon-stimulated gene 15 antibody, Mouse interferon-induced 17 kDa protein antibody, Mouse ubiquitin-like protein ISG15 antibody, Mouse interferon-induced ubiquitin-like protein antibody

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