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Home >> Antibodies >> IRS2 Antibody / Insulin receptor substrate 2

IRS2 Antibody / Insulin receptor substrate 2 (RQ7181)

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  Catalog No Formulation Size Price (USD)  
Image RQ7181 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human HeLa cells with IRS2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Immunofluorescent staining of FFPE human HeLa cells with IRS2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human liver cancer tissue with IRS2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human HeLa cell lysate with IRS2 antibody. Predicted molecular weight ~137 kDa but it may be observed at up to ~190 kDa due to phosphorylation.
Flow cytometry testing of human 293T cells with IRS2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= IRS2 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9Y4H2
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This IRS2 antibody is available for research use only.
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Description

Insulin receptor substrate 2 (IRS2) is a protein that in humans is encoded by the IRS2 gene. It is mapped to 13q34. This gene encodes the insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. The product of this gene is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment. IRS2 is expressed in many cells, it may be essential for signaling by several receptor systems.

Application Notes

Optimal dilution of the IRS2 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids E538-E1338) was used as the immunogen for the IRS2 antibody.

Storage

After reconstitution, the IRS2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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