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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Integrin Beta 1 Antibody / CD29 Polyclonal Antibody (R31512)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
R31512 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 449 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Antigen affinity purified |
| Host | Rabbit |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide |
| Gene ID | 3688 |
| Localization | Cell membrane, cytoplasm |
| Applications | Western Blot : 0.5-1ug/ml IHC (FFPE) : 0.5-1ug/ml |
| Limitations | This Integrin Beta 1 Antibody / CD29 Polyclonal Antibody is available for research use only. |
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Integrin Beta 1 Antibody recognizes integrin beta 1, a transmembrane adhesion receptor subunit encoded by the ITGB1 gene and commonly designated CD29. Integrin beta 1 associates with numerous alpha integrin subunits to form heterodimeric receptors with different ligand-binding properties. These beta 1-containing integrins mediate interactions between cells and extracellular matrix components and contribute to adhesion, migration, signaling, and tissue organization.
Integrin beta 1 is a type I transmembrane glycoprotein containing a large extracellular region, a single transmembrane segment, and a relatively short cytoplasmic tail. Pairing with different alpha subunits produces receptors capable of recognizing extracellular matrix proteins including fibronectin, collagen, and laminin. CD29 also serves as the common beta subunit of several integrins historically designated very late antigen (VLA) receptors.
ITGB1-containing integrins transmit information bidirectionally across the plasma membrane. Inside-out signaling regulates integrin affinity and ligand binding, while extracellular ligand engagement initiates outside-in signals through interactions with cytoskeletal and signaling proteins. These mechanisms influence cell spreading, migration, proliferation, differentiation, survival, and cellular responses to the mechanical properties of the extracellular environment.
Integrin beta 1 is broadly expressed in epithelial, endothelial, stromal, hematopoietic, and other cell populations. Altered ITGB1 expression and signaling are studied in cancer because beta 1-containing integrins can influence tumor cell adhesion, migration, invasion, survival, and interactions with the tumor microenvironment. CD29 is also investigated in immune cell trafficking, tissue development, wound repair, stem cell biology, and cell-matrix communication.
Integrin beta 1 undergoes extensive post-translational glycosylation. Although the unmodified polypeptide has a predicted molecular weight of approximately 88 kDa, mature glycosylated forms can migrate substantially higher during electrophoresis. This biochemical property is relevant when evaluating ITGB1 protein expression by western blot.
An Integrin Beta 1 Antibody can be used to investigate expression of the ITGB1-encoded adhesion receptor subunit, while a CD29 Polyclonal Antibody provides a polyclonal reagent for studies using its established CD designation.
For an additional monoclonal reagent targeting integrin beta 1, see our ITGB1 Antibody ITGB1/3613 / CD29 Monoclonal Antibody page.
The stated application concentrations are suggested starting amounts. Titration of the Integrin Beta 1 Antibody / CD29 Polyclonal Antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Human partial recombinant protein (AA 527-728) was used as the immunogen for this Integrin beta 1 antibody.
After reconstitution, the Integrin beta 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
ITGB1 antibody, CD29 antibody, Fibronectin Receptor Beta Subunit antibody, VLA Beta Chain antibody, Very Late Antigen Beta Chain antibody
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Citations(1)
![Goat Polyclonal CD29 Antibody WB. Western blot testing of gastrointestinal cancer [A] and gall bladder cancer [B] lysates with CD29 antibody at 0.5ug/ml. Predicted molecular weight: 88/130~150 kDa (unmodified/glycosylated).](https://www.nsjbio.com/plp/thumb_image/img-R34833-100UG-1.jpg?v=1791673353 "Goat Polyclonal CD29 Antibody WB. Western blot testing of gastrointestinal cancer [A] and gall bladder cancer [B] lysates with CD29 antibody at 0.5ug/ml. Predicted molecular weight: 88/130~150 kDa (unmodified/glycosylated).")
