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Home >> Antibodies >> IL2R Alpha Antibody / CD25 Antibody

IL2R Alpha Antibody / CD25 Antibody (RQ7081)

  Catalog No Formulation Size Price (USD)  
Image RQ7081 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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IL2R Alpha Antibody in Mouse RAW264.7 Macrophage Cells IF. Immunofluorescence staining of FFPE mouse RAW264.7 macrophage cells using IL2R Alpha Antibody demonstrates distinct green staining associated predominantly with the cell periphery and cytoplasm, while nuclei are counterstained blue with DAPI. HIER was performed by steaming samples in pH 6 citrate buffer for 20 min. The localized cellular fluorescence demonstrates detection of IL2RA by this CD25 Antibody in mouse macrophage cells.
IL2R Alpha Antibody in Mouse Thymus IHC. Immunohistochemistry of FFPE mouse thymus tissue using IL2R Alpha Antibody demonstrates strong staining in a scattered subset of cells against a largely negative lymphoid background. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The discrete distribution of positive cells demonstrates detection of IL2RA by this CD25 Antibody in mouse thymus.
IL2R Alpha Antibody in Rat Thymus IHC. Immunohistochemistry of FFPE rat thymus tissue using IL2R Alpha Antibody demonstrates prominent staining concentrated in a network-like population of cells within the lymphoid tissue, with comparatively little signal in surrounding cells. HIER was performed by boiling tissue sections in pH 8 EDTA for 20 min followed by cooling before testing. The distinct staining pattern demonstrates detection of IL2RA by this CD25 Antibody in rat thymus.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P01590
Localization Cell surface, Cytoplasmic
Applications Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This IL2R Alpha Antibody is available for research use only.
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Description

IL2R Alpha Antibody recognizes CD25, the alpha subunit of the interleukin 2 receptor encoded by the IL2RA gene. CD25 is a type I transmembrane glycoprotein involved in cellular responses to IL-2 and is prominently associated with activated lymphocytes and regulatory T cells. Expression of CD25 increases cellular sensitivity to IL-2 and contributes to mechanisms regulating lymphocyte proliferation, survival, differentiation, and immune homeostasis.

CD25 can bind IL-2 independently as a low-affinity receptor but does not possess the intracellular signaling machinery required to transmit the cytokine signal by itself. Association of CD25 with IL2RB (CD122) and the common gamma chain IL2RG (CD132) produces the high-affinity IL-2 receptor complex. Formation of this receptor allows efficient IL-2 signaling through pathways including JAK-STAT, PI3K-AKT, and MAPKs, linking cytokine recognition to changes in immune-cell function.

IL2RA expression is induced following activation of conventional T and B lymphocytes and is characteristically high on regulatory T cells. CD25-mediated access to IL-2 is particularly important for regulatory T-cell development, maintenance, and suppressive activity. The protein is therefore studied in lymphocyte activation, immune tolerance, inflammatory responses, autoimmunity, and mechanisms regulating adaptive immunity. Proteolytic processing can also generate soluble CD25, providing an additional form associated with immune activation.

This rabbit polyclonal antibody was generated against recombinant mouse IL2RA corresponding to amino acids 22-268. As a CD25 Antibody, it provides a reagent for investigating the alpha subunit of the IL-2 receptor and its relationship to immune-cell activation and cytokine responsiveness. NSJ Bioreagents supplies this antibody for research use only.

An IL2R Alpha Antibody can be used to investigate CD25 expression and molecular mechanisms connecting IL-2 receptor biology, lymphocyte activation, regulatory T-cell function, and immune regulation.

For a monoclonal clone with IHC and protein microarray specificity validation, see our CD25 Antibody IL2RA/2395 / IL2RA Antibody page.

Application Notes

Optimal dilution of the IL2R Alpha Antibody should be determined by the researcher.

Immunogen

Recombinant mouse protein (amino acids E22-I268) was used as the immunogen for the IL2R Alpha Antibody.

Storage

After reconstitution, the IL2R Alpha Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Alternate Names

CD25 antibody, IL2RA antibody, IL-2RA antibody, IL2R alpha antibody, IL-2R alpha antibody, interleukin 2 receptor alpha antibody, interleukin-2 receptor alpha antibody, interleukin 2 receptor alpha chain antibody, TAC antigen antibody, p55 antibody

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