IFI16 Antibody 2I3D7 / PYHIN2 Antibody [clone 2I3D7] (RQ7639)

IFI16 Antibody 2I3D7 by IF. Immunofluorescence staining of FFPE human U-251 cells using mouse monoclonal IFI16 antibody, clone 2I3D7. PYHIN2 immunoreactivity is detected predominantly in cell nuclei (red), with DAPI counterstaining (blue) identifying nuclear DNA. Heat-induced epitope retrieval was performed by steaming in citrate buffer (pH 6.0) for 20 minutes. The overlapping nuclear staining patterns are consistent with the expected predominantly nuclear localization of IFI16.

IFI16 Antibody 2I3D7 by WB. Western blot analysis of human Daudi (lane 1), Raji (lane 2), MOLT4 (lane 3), and U-87 MG (lane 4) cell lysates using mouse monoclonal IFI16 antibody, clone 2I3D7. The antibody detects a prominent band at approximately 85-95 kDa across all four samples, consistent with the expected molecular weight range of IFI16 isoforms. The Daudi sample produces the strongest signal, while additional faint bands are visible in some lanes. These results demonstrate recognition of PYHIN2 in multiple human cell lines.

IFI16 Antibody 2I3D7 by FACS. Flow cytometry analysis of human U-251 cells using mouse monoclonal IFI16 antibody, clone 2I3D7, at 1 ug per million cells. The PYHIN2 antibody-stained population (blue) exhibits a rightward fluorescence shift relative to the isotype control (green) and unstained cells (red). Cells were blocked with goat serum before antibody incubation. The fluorescence separation demonstrates detectable IFI16-associated staining under the tested conditions.