IBA1 Antibody / AIF1 Microglia Marker Antibody [clone AIF1/1909] (V3830)

IBA1 Antibody Human Lymph Node IHC. Immunohistochemistry analysis of FFPE human lymph node tissue using IBA1 Antibody (clone AIF1/1909) shows strong HRP-DAB brown cytoplasmic staining in macrophages and dendritic-like cells within paracortical and sinusoidal regions, highlighting AIF1 / IBA1 expression in tissue-resident immune cells that share functional lineage with microglia, consistent with its role as a microglia marker and macrophage activation marker; nuclei are counterstained blue. HIER: boil tissue sections in pH 6 citrate buffer for 10-20 min and allow to cool before testing.

IBA1 Antibody Human Kidney IHC. Immunohistochemistry analysis of FFPE human kidney tissue using IBA1 Antibody (clone AIF1/1909) demonstrates HRP-DAB brown cytoplasmic staining in interstitial macrophages and mononuclear cells surrounding renal tubules and glomeruli, while tubular epithelial cells remain largely negative, highlighting AIF1 / IBA1 expression in tissue-resident macrophages that are functionally analogous to microglia, consistent with its role as a microglia marker and immune activation marker; nuclei are counterstained blue. HIER: boil tissue sections in pH 6 citrate buffer for 10-20 min and allow to cool before testing.

IBA1 Antibody Human Tonsil IHC. Immunohistochemistry analysis of FFPE human tonsil tissue using IBA1 Antibody (clone AIF1/1909) shows HRP-DAB brown cytoplasmic staining in scattered macrophages and dendritic-like cells within germinal centers and interfollicular regions, while lymphocytes remain largely negative, highlighting AIF1 / IBA1 expression in immune cell populations that share functional lineage with microglia, consistent with its role as a microglia marker and macrophage activation marker; nuclei are counterstained blue. HIER: boil tissue sections in pH 6 citrate buffer for 10-20 min and allow to cool before testing.

IBA1 Antibody Microarray Specificity Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using IBA1 Antibody (clone AIF1/1909) demonstrates highly specific detection of AIF1 / IBA1, a microglia marker and macrophage lineage protein. The antibody shows a dominant signal for AIF1 with a Z-score of 94.75 and an S-score of 81.96, indicating strong separation from all other proteins on the array and supporting high target specificity. Z- and S-score explanation: The Z-score reflects the strength of signal generated when the antibody binds to a protein on the array, expressed as standard deviations above the mean signal, while the S-score represents the difference between sequential Z-scores and indicates relative specificity compared to potential off-target interactions.

SDS-PAGE analysis of purified, BSA-free IBA1 antibody clone AIF1/1909 as confirmation of integrity and purity.