IBA1 Antibody - HuProt Validated / AIF1 [clone AIF1/1909] (V3830)

Immunohistochemistry of IBA1 antibody in human lymph node. Formalin-fixed, paraffin-embedded human lymph node tissue shows strong cytoplasmic HRP-DAB brown staining in macrophages and dendritic-like cells within the paracortical and sinusoidal regions. Staining was performed using clone AIF1/1909, a HuProt validated antibody, following heat-induced epitope retrieval by boiling sections in pH 6 citrate buffer for 10-20 min and cooling at room temperature for 20 min. The staining pattern is consistent with expected IBA1 expression in tissue-resident macrophages.

Immunohistochemistry of IBA1 antibody in human kidney tissue. Formalin-fixed, paraffin-embedded human kidney shows cytoplasmic HRP-DAB brown staining in interstitial macrophages and mononuclear cells surrounding renal tubules and glomeruli, while tubular epithelial cells remain largely negative. Staining was performed using clone AIF1/1909, a HuProt validated antibody, following heat-induced epitope retrieval by boiling sections in pH 6 citrate buffer for 10-20 min and cooling at room temperature for 20 min. The staining distribution is consistent with expected IBA1 expression in tissue-resident macrophages.

Immunohistochemistry of IBA1 antibody in human tonsil. Formalin-fixed, paraffin-embedded human tonsil tissue shows cytoplasmic HRP-DAB brown staining in scattered macrophages and dendritic-like cells within the interfollicular regions and germinal centers, with lymphocytes largely negative. Staining was performed using clone AIF1/1909, a HuProt validated antibody, following heat-induced epitope retrieval by boiling sections in pH 6 citrate buffer for 10-20 min and cooling at room temperature for 20 min. The staining pattern is consistent with expected IBA1 expression in tissue-resident macrophages.

Human protein microarray specificity validation of IBA1 antibody. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins was performed using IBA1 antibody HuProt validated clone AIF1/1909. The antibody demonstrates strongest binding to AIF1 with a Z-score of 94.75 and an S-score of 81.96, indicating clear separation from all other proteins on the array. These results support the high specificity of clone AIF1/1909 for its intended target.
Z- and S-score explanation: The Z-score represents the strength of signal produced when the antibody, in combination with a fluorescently tagged anti-IgG secondary antibody, binds to a specific protein on the HuProt(TM) array. Z-scores are expressed in standard deviations above the mean signal of all proteins on the array. Proteins are ranked in descending order based on Z-score. The S-score represents the difference in Z-scores between sequentially ranked proteins and reflects the relative specificity of the antibody for its intended target compared to potential off-target interactions.

SDS-PAGE analysis of purified, BSA-free IBA1 antibody clone AIF1/1909 as confirmation of integrity and purity.