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Home >> Antibodies >> HTRA1 Antibody

HTRA1 Antibody (F54502)

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  Catalog No Formulation Size Price (USD)  
Image F54502-0.2ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.2 ml 399
Image
F54502-0.05ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.05 ml 185
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Immunofluorescent staining of human HeLa cells with HTRA1 antibody (green) and DAPI nuclear stain (blue).
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Immunofluorescent staining of human HeLa cells with HTRA1 antibody (green) and DAPI nuclear stain (blue).
Western blot testing of human HeLa cell lysate with HTRA1 antibody. Predicted molecular weight ~51 kDa.
Western blot testing of mouse brain lysate with HTRA1 antibody. Predicted molecular weight ~51 kDa.
IHC testing of FFPE human kidney tissue with HTRA1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt Q92743
Localization Cytoplasmic, cell membrane
Applications Western blot : 1:500-1:2000
Immunofluorescence : 1:25
Immunohistochemistry (FFPE) : 1:25
Limitations This HTRA1 antibody is available for research use only.
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  • Applications : IHC-P, WB
    Reactivity : Human, Mouse, Rat

Description

HtrA1 is a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth.

Application Notes

The stated application concentrations are suggested starting points. Titration of the HtrA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 116-147 from the human protein was used as the immunogen for the HTRA1 antibody.

Storage

Aliquot the HTRA1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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