HSP105 Antibody / Heat Shock Protein 105 Antibody (RQ4470)

HSP105 Antibody Human U-2 OS Osteosarcoma Cell IF. Immunofluorescence staining of human U-2 OS osteosarcoma cells was performed with HSP105 Antibody, also called Heat Shock Protein 105 Antibody (red), at 5 ug/ml overnight at 4oC. Enzyme antigen retrieval was performed for 15 min followed by blocking with 10% goat serum. Fluoro550-conjugated goat anti-rabbit IgG was used as the secondary antibody, and nuclei were counterstained with DAPI (blue). HSP105/HSPH1 staining is observed predominantly in the cytoplasm, demonstrating Heat Shock Protein 105 localization in human U-2 OS cells.

HSP105 Antibody Human Breast Cancer IHC. Immunohistochemistry staining of FFPE human breast cancer tissue was performed with HSP105 Antibody, also called Heat Shock Protein 105 Antibody, at 2 ug/ml. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8, followed by blocking with 10% goat serum and overnight primary antibody incubation at 4oC. HRP-conjugated secondary antibody and DAB were used for detection. Positive HSP105/HSPH1 staining is observed in tumor cells, demonstrating Heat Shock Protein 105 expression in human breast cancer tissue.

HSP105 Antibody Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue was performed with HSP105 Antibody, also called Heat Shock Protein 105 Antibody, at 2 ug/ml. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8, followed by blocking with 10% goat serum and overnight primary antibody incubation at 4oC. HRP-conjugated secondary antibody and DAB were used for detection. Positive HSP105/HSPH1 staining is observed in cells throughout the brain tissue, demonstrating Heat Shock Protein 105 expression in rat brain.

HSP105 Antibody Human U251 Glioblastoma Cell Immunoprecipitation. Immunoprecipitation of HSP105 was performed from human U251 glioblastoma whole cell lysate using HSP105 Antibody, also called Heat Shock Protein 105 Antibody. Lane 1: 30 ug of U251 input lysate; Lane 2: material immunoprecipitated with rabbit control IgG; Lane 3: material immunoprecipitated with 2 ug of HSP105 Antibody from 500 ug of U251 lysate. Western blot detection was performed with HSP105 Antibody at 0.5 ug/ml followed by HRP-conjugated secondary antibody and ECL detection. HSP105/HSPH1 is detected at approximately 105 kDa, near the expected molecular weight of approximately 97 kDa, demonstrating successful immunoprecipitation of Heat Shock Protein 105 from human U251 cells.

HSP105 Antibody Human Cancer and Stress-Responsive Cell WB. Western blot analysis was performed with HSP105 Antibody, also called Heat Shock Protein 105 Antibody, using human 1) HaCaT, 2) A549, 3) Caco-2, 4) MCF-7, 5) PC-3, 6) SH-SY5Y, 7) U251 and 8) HepG2 whole cell lysates. Samples were loaded at 30 ug per lane, and the antibody was used at 0.5 ug/ml overnight at 4oC. A prominent HSP105/HSPH1 band is detected at approximately 105 kDa across the tested cell lines, near the expected molecular weight of approximately 97 kDa. This HSP105 Antibody demonstrates detection of Heat Shock Protein 105 across diverse human cell lines.

HSP105 Antibody Mouse and Rat Brain, Testis and Cell WB. Western blot analysis was performed with HSP105 Antibody, also called Heat Shock Protein 105 Antibody, using 1) rat testis, 2) rat brain, 3) rat PC-12 cells, 4) rat C6 cells and 5) mouse brain lysates. Samples were loaded at 30 ug per lane, and the antibody was used at 0.5 ug/ml overnight at 4oC. A prominent HSP105/HSPH1 band is detected at approximately 105 kDa across the tested samples, near the expected molecular weight of approximately 97 kDa. This HSP105 Antibody demonstrates detection of Heat Shock Protein 105 in mouse and rat neural and non-neural samples.

HSP105 Antibody Human Cancer and Stress-Responsive Cell Fluorescent WB. Western blot analysis was performed with HSP105 Antibody, also called Heat Shock Protein 105 Antibody, using human 1) HaCaT, 2) A549, 3) Caco-2, 4) MCF-7, 5) PC-3, 6) SH-SY5Y, 7) U251 and 8) HepG2 whole cell lysates. Samples were loaded at 30 ug per lane, and the antibody was used at 0.5 ug/ml overnight at 4oC followed by a Fluoro647-conjugated secondary antibody. A prominent HSP105/HSPH1 band is detected at approximately 105 kDa, near the expected molecular weight of approximately 97 kDa. This HSP105 Antibody demonstrates fluorescent western blot detection of Heat Shock Protein 105 across diverse human cell lines.