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Home >> Antibodies >> HER2 Antibody

HER2 Antibody (F50602)

  Catalog No Formulation Size Price (USD)  
Image F50602-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 359
Image
F50602-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot analysis of HER2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ErbB2 gene (2).
IHC analysis of FFPE human breast carcinoma tissue stained with HER2 antibody
Flow cytometric analysis of MCF-7 cells using HER2 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of ErbB2/ HER2 antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P04626
Applications Western blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This HER2 antibody is available for research use only.
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Description

ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.

Application Notes

Titration of the HER2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This HER2 antibody was produced from rabbits immunized with human recombinant ErbB2 protein.

Storage

Aliquot the HER2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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