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Home >> Antibodies >> HER2 Antibody

HER2 Antibody (F50602)

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  Catalog No Formulation Size Price (USD)  
Image F50602-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F50602-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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Western blot analysis of HER2 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ErbB2 gene (2).
HER2 Antibody Breast Carcinoma IHC. Immunohistochemistry analysis of FFPE human breast carcinoma tissue stained with HER2 antibody.
Flow cytometric analysis of MCF-7 cells using HER2 antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Confocal immunofluorescent analysis of ErbB2 / HER2 antibody with MCF-7 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P04626
Applications Western Blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Flow Cytometry : 1:10-1:50
Immunofluorescence : 1:10-1:50
Limitations This HER2 antibody is available for research use only.
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Description

ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.

For broad detection of HER2 (ErbB2) as a receptor tyrosine kinase, see our HER2 antibody.

Application Notes

Titration of the HER2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This HER2 antibody was produced from rabbits immunized with human recombinant ErbB2 protein.

Storage

Aliquot the HER2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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