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Home >> Antibodies >> GSDMD Antibody / Gasdermin D

GSDMD Antibody / Gasdermin D [clone 6D11] (RQ7955)

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  Catalog No Formulation Size Price (USD)  
Image RQ7955 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 449
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Immunofluorescent staining of FFPE human PC-3 cells with GSDMD antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human esophageal squamous carcinoma tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical intraepithelial neoplasia tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human cervical cancer tissue with GSDMD antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) ThP-1, 2) SW620, 3) Jurkat, 4) U-87 MG and 5) PC-3 cell lysate with GSDMD antibody. Predicted molecular weight ~53 kDa.
Flow cytometry testing of human Jurkat cells with GSDMD antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= GSDMD antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Mouse
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b
Clone Name 6D11
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P57764
Localization Nucleus, cytoplasm, cell membrane, secreted
Applications Western Blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 2-5ug/ml
Immunofluorescence (FFPE) : 5ug/ml
Flow Cytometry : 1-3ug/million cells
Limitations This GSDMD antibody is available for research use only.
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Description

Gasdermin D is a precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals. [UniProt]

Application Notes

Optimal dilution of the GSDMD antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids M1-H484) was used as the immunogen for the GSDMD antibody.

Storage

After reconstitution, the GSDMD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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