CD235a Antibody ADOG-7 / Erythroid Marker Antibody [clone ADOG-7] (RQ5004)

CD235a Antibody in Human K562 Erythroleukemia Cells WB. Western blot testing of CD235a Antibody ADOG-7 / Erythroid Marker Antibody in human K562 erythroleukemia cell lysate. The antibody was used at a 1:1,000 dilution for 1 hour at RT. A prominent band is observed at approximately 38-40 kDa, consistent with the elevated apparent molecular weight of heavily glycosylated glycophorin A. This CD235a Antibody demonstrates detection of GYPA in erythroid-lineage K562 cells.

CD235a Antibody in Human Fetal Liver WB. Western blot testing of CD235a Antibody ADOG-7 / Erythroid Marker Antibody in human fetal liver lysate. A strong, discrete band is observed at approximately 38 kDa. Glycophorin A has a calculated polypeptide molecular weight of approximately 16.3 kDa but migrates at a substantially higher apparent molecular weight due to its extensive glycosylation. This CD235a Antibody demonstrates detection of the mature GYPA glycoprotein in human fetal liver.

CD235a Antibody in Rat Kidney WB. Western blot testing of CD235a Antibody ADOG-7 / Erythroid Marker Antibody in rat kidney lysate. The antibody was used at a 1:1,000 dilution for 1 hour at RT. A strong, discrete band is observed at approximately 40-42 kDa, with minimal additional background. The observed band indicates recognition of a glycophorin A-related protein in the tested rat kidney lysate.

CD235a Antibody in Human Colon Cancer IHC. Immunohistochemistry testing of CD235a Antibody ADOG-7 / Erythroid Marker Antibody in FFPE human colon cancer tissue. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:100 overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Strong staining is observed predominantly in erythrocytes within vascular spaces, while the surrounding colonic epithelial cells show little staining.

CD235a Antibody in Human Lung Cancer IHC. Immunohistochemistry testing of CD235a Antibody ADOG-7 / Erythroid Marker Antibody in FFPE human lung cancer tissue. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8.0, followed by blocking with 10% goat serum. The antibody was used at 1:100 overnight at 4oC, followed by peroxidase-conjugated goat anti-rabbit IgG and DAB detection. Strong staining is concentrated in numerous small cells consistent with erythrocytes, while the larger tumor epithelial cells show comparatively little immunoreactivity.

CD235a Antibody in Human Lung Cancer Tissue IHC. Immunohistochemistry testing of CD235a Antibody ADOG-7 / Erythroid Marker Antibody in a second FFPE human lung cancer tissue section. Heat-induced epitope retrieval was performed in EDTA buffer, pH 8.0, and the antibody was used at 1:100 overnight at 4oC. Widespread immunoreactivity is visible throughout the tissue, including numerous small cells within the tumor-associated stroma.