Glutamine Synthetase Antibody for WB / GLUL [clone CCH-7] (RQ5441)

Western blot testing of human 1) Jurkat and 2) HeLa cell lysate with Glutamine Synthetase antibody for WB. Predicted molecular weight ~42 kDa.

Western blot analysis of Glutamine Synthetase Antibody for WB CCH-7 in multiple cell and tissue lysates. Lane 1: 293 cells, Lane 2: BxPC-3 cells, Lane 3: mouse liver, Lane 4: rat liver, Lane 5: rat spleen, Lane 6: rat kidney, Lane 7: mouse stomach. A band is detected at approximately 42 kDa, consistent with the predicted molecular weight of Glutamate-ammonia ligase (GLUL). Strong signal is observed in liver samples, aligning with the known pericentral hepatocyte enrichment of glutamine synthetase, while lower intensity bands are present in non-hepatic tissues. The antibody was used at a 1:5,000 dilution for 1 hour at room temperature.

IHC staining of FFPE human liver with Glutamine Synthetase antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Immunohistochemistry of Glutamine Synthetase Antibody CCH-7 in human glioblastoma. Formalin-fixed, paraffin-embedded human glioblastoma tissue demonstrates diffuse cytoplasmic HRP-DAB brown staining in tumor cells, consistent with Glutamate-ammonia ligase (GLUL) expression in astrocytic lineage-derived cells. Background stromal elements show comparatively lower signal. The antibody was used at a 1:250 dilution for paraffin-embedded tissue analysis.

Immunohistochemistry of Glutamine Synthetase Antibody CCH-7 in human prostate carcinoma. Formalin-fixed, paraffin-embedded human prostate cancer tissue demonstrates cytoplasmic HRP-DAB brown staining in malignant epithelial cells, with variable intensity across tumor regions. Adjacent stromal components show comparatively weaker signal. Staining is consistent with Glutamate-ammonia ligase (GLUL) cytoplasmic localization. The antibody was applied at a 1:250 dilution for paraffin-embedded tissue analysis.

Immunohistochemistry of Glutamine Synthetase Antibody in rat cerebral cortex. Formalin-fixed, paraffin-embedded rat cortex demonstrates diffuse cytoplasmic HRP-DAB brown staining in astrocytic cells distributed throughout the neuropil, consistent with Glutamate-ammonia ligase (GLUL) localization in glial populations. Neuronal cell bodies show comparatively weaker staining, while background is minimal. The antibody was applied at a 1:400 dilution for paraffin-embedded tissue analysis.

Immunohistochemistry of Glutamine Synthetase Antibody in rat kidney. Formalin-fixed, paraffin-embedded rat kidney tissue demonstrates cytoplasmic HRP-DAB brown staining in renal tubular epithelial cells, consistent with Glutamate-ammonia ligase (GLUL) expression in metabolically active nephron segments. Glomerular structures show comparatively lower staining intensity. The antibody was applied at a 1:400 dilution for paraffin-embedded tissue analysis.

Immunohistochemistry of Glutamine Synthetase Antibody in mouse ovary. Formalin-fixed, paraffin-embedded mouse ovarian tissue demonstrates cytoplasmic HRP-DAB brown staining in granulosa cells and stromal cell populations, consistent with Glutamate-ammonia ligase (GLUL) localization in metabolically active ovarian compartments. Oocyte nuclei remain unstained, while surrounding follicular cells show diffuse cytoplasmic signal. The antibody was applied at a 1:400 dilution for paraffin-embedded tissue analysis.

Immunofluorescence of Glutamine Synthetase Antibody in HeLa cells. HeLa cells show weak cytoplasmic fluorescence (green) consistent with Glutamate-ammonia ligase (GLUL) localization, while nuclei are counterstained with DAPI (blue). Phalloidin-TRITC (red) highlights actin filaments. The merged image demonstrates faint cytoplasmic GLUL staining surrounding the nuclei without nuclear overlap. The Glutamine Synthetase Antibody was used at a 1:50 dilution.