GLUT1 Immunofluorescence Antibody / SLC2A1 Glucose Transporter [clone GLUT1/2476] (V3920)

IHC testing of human bladder with GLUT1 antibody (clone GLUT1/2476). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of human breast carcinoma with GLUT1 antibody (clone GLUT1/2476). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

GLUT1 Immunofluorescence Antibody staining in human K562 cells. Fluorescent staining shows green signal corresponding to GLUT1 (SLC2A1) localization outlining the plasma membrane of individual cells, consistent with membrane-associated glucose transporter distribution. The membrane fluorescence delineates cell boundaries while weaker cytoplasmic fluorescent signal reflects intracellular transporter pools. Nuclei are counterstained with Reddot nuclear stain (red). Signal was detected using an immunofluorescence method and visualized by fluorescence microscopy.

Western blot testing of human A431 cells with GLUT1 antibody (clone GLUT1/2476). Expected molecular weight: 45-55 kDa.

Flow cytometry testing of human K562 cells with GLUT1 antibody (clone GLUT1/2476); Red=isotype control, Blue= GLUT1 antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GLUT1 antibody (clone GLUT1/2476). These results demonstrate the foremost specificity of the GLUT1/2476 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free GLUT1 antibody (clone GLUT1/2476) as confirmation of integrity and purity.