GLUT1 Antibody Protein Microarray Validated / SLC2A1 Glucose Transporter [clone GLUT1/2475] (V3919)

GLUT1 Antibody Protein Microarray Validated clone GLUT1/2475 immunohistochemistry analysis in human bladder carcinoma tissue. FFPE human bladder carcinoma section shows strong HRP-DAB brown membranous and cytoplasmic staining in tumor epithelial cells, consistent with GLUT1 (SLC2A1) localization in metabolically active cancer cell populations with elevated glucose uptake. Tumor cell nests display prominent membrane-associated staining while surrounding stromal regions show minimal background signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9, 10 mM Tris with 1 mM EDTA for 10-20 minutes followed by cooling at room temperature prior to antibody staining.

IHC testing of human tongue with GLUT1 antibody (clone GLUT1/2475). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

GLUT1 Antibody Protein Microarray Validated clone GLUT1/2475 immunofluorescence staining in human K562 cells. Fluorescent staining shows green signal corresponding to GLUT1 (SLC2A1) localization outlining the plasma membrane and cytoplasmic vesicular structures consistent with glucose transporter distribution in metabolically active cells. Membrane-associated fluorescence highlights cell boundaries while punctate cytoplasmic signal reflects intracellular transporter trafficking. Nuclei are counterstained with Reddot nuclear stain (red), and signal was detected using an immunofluorescence method and visualized by fluorescence microscopy.

Western blot testing of human A431 cells with GLUT1 antibody (clone GLUT1/2475). Expected molecular weight: 45-55 kDa.

Flow cytometry testing of human K562 cells with GLUT1 antibody (clone GLUT1/2475); Red=isotype control, Blue= GLUT1 antibody.

GLUT1 Antibody Protein Microarray Validated (clone GLUT1/2475) specificity analysis using the HuProt human protein microarray platform. The HuProt array contains more than 19,000 full-length human proteins and enables large-scale evaluation of antibody binding across the human proteome. In this assay, clone GLUT1/2475 shows the strongest signal for GLUT1 (SLC2A1), indicating highly selective target recognition relative to other proteins present on the array. Z-score represents the strength of antibody binding to a specific protein expressed as standard deviations above the mean signal of the entire array, while the S-score represents the difference between the top-ranked Z-score and the next highest signal, providing a measure of antibody target specificity. High Z-score and S-score values for GLUT1 demonstrate strong preferential binding and support the specificity of clone GLUT1/2475 for detecting the GLUT1 glucose transporter.

SDS-PAGE analysis of purified, BSA-free GLUT1 antibody (clone GLUT1/2475) as confirmation of integrity and purity.