GLUT1 Antibody [clone GLUT1/2475] (V3919)

IHC testing of human bladder carcinoma with GLUT1 antibody (clone GLUT1/2475). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of human tongue with GLUT1 antibody (clone GLUT1/2475). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of human K562 cells with GUT1 antibody (green, clone GLUT1/2475) and Reddot nuclear stain (red).

Western blot testing of human A431 cells with GLUT1 antibody (clone GLUT1/2475). Expected molecular weight: 45-55 kDa.

Flow cytometry testing of human K562 cells with GLUT1 antibody (clone GLUT1/2475); Red=isotype control, Blue= GLUT1 antibody.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using GLUT1 antibody (clone GLUT1/2475). These results demonstrate the foremost specificity of the GLUT1/2475 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free GLUT1 antibody (clone GLUT1/2475) as confirmation of integrity and purity.