• Tel: 858.663.9055
  • SeparatorEmail: info@nsjbio.com
  • Tel: 858.663.9055
  • Email: info@nsjbio.com
Home >> Antibodies >> Glucosidase 2 subunit beta Antibody / PRKCSH

Glucosidase 2 subunit beta Antibody / PRKCSH (RQ8021)

  Catalog No Formulation Size Price (USD)  
Image RQ8021 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
Bulk quote request
Immunofluorescent staining of FFPE human A549 cells with Glucosidase 2 subunit beta antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human 293T, 3) human Daudi, 4) human HEL, 5) rat liver, 6) rat L6, 7) mouse liver and 8) mouse C2C12 cell lysate with Glucosidase 2 subunit beta antibody. Predicted molcular weight ~59 kDa but may be observed at higher molecular weights due to glycosylation.
Flow cytometry testing of fixed and permeabilized human SiHa cells with Glucosidase 2 subunit beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Glucosidase 2 subunit beta antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P14314
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Immunofluorescence : 5ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This Glucosidase 2 subunit beta antibody is available for research use only.
Review this product on BioCompare and get a $20 Amazon gift card

Description

Glucosidase 2 subunit beta is an enzyme that in humans is encoded by the PRKCSH gene. This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the Glucosidase 2 subunit beta antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids E16-L506) was used as the immunogen for the Glucosidase 2 subunit beta antibody.

Storage

After reconstitution, the Glucosidase 2 subunit beta antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Cross
Bulk Quote Request Form
Name*:
Organization*:
Email*:
Phone Number*:
Catalog No.*:
Comments and Specifics(amount, formulation, etc.)*:
Validation code: Captchapackage Image


Can't read the image? click here to refresh.
    *required field

Your bulk quote request has been submitted successfully!

Please contact us if you have any questions.