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Home >> Antibodies >> Glucose-6-phosphate isomerase Antibody / GPI

Glucose-6-phosphate isomerase Antibody / GPI [clone GPI/2439] (V5718)

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  Catalog No Formulation Size Price (USD)  
Image V5718-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 100 ug 539
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V5718-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide 20 ug 249
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V5718SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 539
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IHC staining of FFPE human hepatocellular carcinoma tissue with Glucose-6-phosphate isomerase antibody (clone GPI/2439). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human hepatocellular carcinoma tissue with Glucose-6-phosphate isomerase antibody (clone GPI/2439). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human kidney tissue with Glucose-6-phosphate isomerase antibody (clone GPI/2439). Predicted molecular weight ~63 kDa.
SDS-PAGE analysis of purified, BSA-free Glucose-6-phosphate isomerase antibody (clone GPI/2439) as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name GPI/2439
Purity Protein G affinity
UniProt P06744
Localization Cytoplasm
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Western Blot : 2-4ug/ml
Limitations This Glucose-6-phosphate isomerase antibody is available for research use only.
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Description

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency.

Application Notes

Optimal dilution of the Glucose-6-phosphate isomerase antibody should be determined by the researcher.

Immunogen

A recombinant human full-length GPI protein was used as the immunogen for the Glucose-6-phosphate isomerase antibody.

Storage

Aliquot the Glucose-6-phosphate isomerase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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