GK2 Antibody / Sperm Mitochondrial Metabolism Antibody (FY12204)

GK2 Antibody MG-63 Cell IF. Immunofluorescent staining of cultured MG-63 cells using GK2 Antibody (green) following enzyme-mediated antigen retrieval and DAPI nuclear counterstaining (blue). GK2 displays predominantly cytoplasmic staining with a punctate distribution consistent with mitochondrial localization, supporting its proposed role in sperm mitochondrial metabolism and energy production. This result demonstrates the utility of GK2 Antibody, a Sperm Mitochondrial Metabolism Antibody, for investigating GK2 localization, mitochondrial biology and male reproductive function in cultured cells.

GK2 Antibody Human, Mouse and Rat WB. Western blot analysis of GK2 Antibody using lysates from lane 1: human RT4 cells, lane 2: human U251 cells, lane 3: human U-87 MG cells, lane 4: human SH-SY5Y cells, lane 5: rat testis, lane 6: rat brain, lane 7: mouse testis and lane 8: mouse brain. A specific band is detected at approximately 61 kDa in all samples, consistent with the predicted molecular weight of endogenous GK2. These results demonstrate cross-species detection of GK2 and support the use of GK2 Antibody, a Sperm Mitochondrial Metabolism Antibody, for studies of mitochondrial function, spermatogenesis and male reproductive biology.

GK2 Antibody Human SH-SY5Y FACS. Flow cytometry analysis of fixed and permeabilized SH-SY5Y human neuroblastoma cells stained with GK2 Antibody at 1 ug/10^6 cells, followed by a DyLight 488-conjugated secondary antibody. The blue histogram (GK2) exhibits a clear rightward shift relative to the rabbit IgG isotype control (green) and unstained cells (red), demonstrating specific intracellular detection of endogenous GK2. These results support the use of GK2 Antibody, a Sperm Mitochondrial Metabolism Antibody, for analyzing GK2 expression, mitochondrial biology and reproductive protein function by flow cytometry.