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Home >> Antibodies >> GAPDH Antibody

GAPDH Antibody (R34148)

  Catalog No Formulation Size Price (USD)  
Image R34148-100UG 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide 100 ug 429
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Western blot testing of 1) human HeLa and 2) mouse NIH3T3 lysate with GAPDH antibody at 0.03ug/ml. Predicted molecular weight ~36 kDa.
Western blot testing of human 1) liver, 2) testis and 3) tonsil lysate with GAPDH antibody at 0.1/0.03/0.03 ug/ml, respectively. Predicted molecular weight ~36 kDa.
Western blot testing of 1) mouse liver and 2) rat heart lysate with GAPDH antibody at 0.1 and 0.03 ug/ml, respectively. Predicted molecular weight ~36 kDa.
IHC staining of FFPE human liver with GAPDH antibody at 2ug/ml. HIER: steamed with pH6 citrate buffer, HRP-staining.
IF/ICC testing of fixed and permeabilized human HeLa cells with GAPDH antibody (green) at 5ug/ml and DAPI nuclear stain (blue).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Predicted Reactivity Dog
Format Antigen affinity purified
Clonality Polyclonal (goat origin)
Isotype Goat Ig
Purity Antigen affinity
Gene ID 2597
Localization Cytoplasmic, nuclear
Applications Western blot : 0.1-0.3ug/ml
Immunohistochemistry (FFPE) : 2-4ug/ml
IF/ICC : 5ug/ml
ELISA (peptide) LOD : 1:16000
Limitations This GAPDH antibody is available for research use only.
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Description

Additional name(s) for this target protein: Glyceraldehyde-3-phosphate dehydrogenase

Application Notes

Optimal dilution of the GAPDH antibody should be determined by the researcher.

Immunogen

Amino acids GVNHEKYDNSLK were used as the immunogen for this GAPDH antibody.

Storage

Aliquot and store the GAPDH antibody at -20oC.

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