GADD34 Antibody / Stress Response Protein Antibody (R33103)

GADD34 Antibody Human Liver Cancer Cell WB. Western blot analysis of GADD34 expression in human HEPG2 and KLY cell lysates. Lane 1: HEPG2 cell lysate. Lane 2: KLY negative control cell lysate. Proteins were detected using GADD34 antibody at 0.1 ug/ml. A distinct band is observed at approximately 73 kDa in HEPG2 cells, consistent with the predicted molecular weight of GADD34, while no corresponding band is detected in the negative control lysate. GADD34 may also be detected at higher apparent molecular weights of up to approximately 110 kDa due to post-translational modification or altered protein migration. This result demonstrates the specificity of the GADD34 Antibody / Stress Response Protein Antibody for detecting endogenous GADD34 by Western blot.

GADD34 Antibody Human Liver IHC. Immunohistochemical staining of FFPE human liver using GADD34 antibody at 2 ug/ml following heat-induced epitope retrieval in pH6 citrate buffer. Detection was performed using an HRP-based staining system. GADD34 immunoreactivity is observed predominantly within hepatocytes, with diffuse cytoplasmic staining and variable staining intensity throughout the liver parenchyma, consistent with the protein's role in regulating cellular stress responses and recovery from translational inhibition. This staining demonstrates that the GADD34 Antibody / Stress Response Protein Antibody is suitable for immunohistochemical detection of endogenous GADD34 in formalin-fixed, paraffin-embedded human tissue.

GADD34 Antibody Human Liver Cancer Cell IF. Immunofluorescent staining of fixed and permeabilized human HepG2 cells using GADD34 antibody at 10 ug/ml (green) with DAPI nuclear counterstain (blue). GADD34 immunoreactivity is detected predominantly within the cytoplasm with additional perinuclear staining, consistent with its role in regulating protein phosphatase 1-mediated dephosphorylation of eIF2alpha during the integrated stress response. The negative control shows minimal background fluorescence, supporting staining specificity. This result demonstrates that the GADD34 Antibody / Stress Response Protein Antibody is suitable for immunofluorescent detection of endogenous GADD34 in cultured human cells.

GADD34 Antibody Human Cervical Cancer Cell IF. Immunofluorescent staining of fixed and permeabilized human HeLa cells using GADD34 antibody at 10 ug/ml (green) with DAPI nuclear counterstain (blue). GADD34 immunoreactivity is detected predominantly within the cytoplasm with prominent perinuclear staining, consistent with its function as a stress-inducible regulator of protein phosphatase 1 and eIF2alpha signaling during the integrated stress response. The negative control exhibits minimal background fluorescence, supporting the specificity of the staining pattern. This result demonstrates that the GADD34 Antibody / Stress Response Protein Antibody is suitable for immunofluorescent detection and subcellular localization of endogenous GADD34 in cultured human cells.

GADD34 Antibody Human Liver Cancer Cell FACS. Flow cytometric analysis of fixed and permeabilized human HepG2 cells stained with GADD34 antibody at 10 ug/ml (blue) or naïve goat Ig as the isotype control (black). GADD34 antibody staining produces a clear rightward shift in fluorescence intensity compared with the control population, demonstrating specific detection of endogenous GADD34 expression. This result supports the use of the GADD34 Antibody / Stress Response Protein Antibody for intracellular flow cytometry applications and evaluation of GADD34 expression in cultured human cells.