FTL Antibody / Ferritin Complex Assembly Antibody [clone FTL/1387] (V7412)

FTL Antibody Prostate IHC. Immunohistochemistry analysis of FFPE human prostate tissue stained with FTL Antibody demonstrates granular cytoplasmic HRP-DAB brown staining within glandular epithelial cell populations, consistent with expression of ferritin light chain / FTL in metabolically active secretory epithelial compartments. This ferritin complex assembly antibody highlights intracellular ferritin-associated iron sequestration pathways and oxidative stress-responsive cellular regulation in prostate tissue. HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allow to cool before testing.

FTL Antibody HeLa WB. Western blot analysis of human HeLa cell lysate using FTL Antibody detects a strong band at approximately 20 kDa, consistent with ferritin light chain / FTL. A weaker higher molecular weight band may represent ferritin complex-associated or partially processed ferritin species. This ferritin complex assembly antibody supports detection of an intracellular ferritin subunit involved in iron sequestration, ferritin nanocage organization, and oxidative stress-responsive cellular regulation pathways.

FTL Antibody HuProt Microarray Validation. Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FTL Antibody demonstrates highly selective recognition of ferritin light chain / FTL with strong separation from non-target proteins across the tested human proteome. This ferritin complex assembly antibody showed the highest signal intensity for FTL, supporting the foremost specificity of clone FTL/1387 in large-scale protein microarray validation studies. In HuProt(TM) analysis, the Z-score represents the strength of antibody binding signal relative to the overall array mean, while the S-score reflects target specificity relative to the next highest ranked protein signal. Elevated S-score separation supports preferential target recognition and reduced off-target reactivity in complex proteomic environments.

SDS-PAGE analysis of purified, BSA-free FTL antibody as confirmation of integrity and purity.

FTL Antibody Pancreas IHC. Immunohistochemistry analysis of FFPE human pancreas tissue stained with FTL Antibody demonstrates strong granular cytoplasmic HRP-DAB brown staining within clustered islet-associated endocrine cell populations, while surrounding exocrine pancreatic tissue shows comparatively lower background reactivity. The staining pattern is consistent with expression of ferritin light chain / FTL in metabolically active pancreatic cellular compartments involved in ferritin complex assembly and intracellular iron sequestration pathways. This ferritin complex assembly antibody highlights ferritin-associated oxidative stress-responsive cellular regulation within pancreatic tissue. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

FTL Antibody Kidney WB. Western blot analysis of human kidney tissue lysate using FTL Antibody detects a strong band at approximately 20 kDa, consistent with ferritin light chain / FTL. Additional diffuse higher molecular weight signal may reflect ferritin complex-associated species or partially assembled ferritin protein complexes present in metabolically active renal tissue. This ferritin complex assembly antibody supports detection of intracellular ferritin-associated iron sequestration pathways and oxidative stress-responsive cellular regulation in kidney tissue.