FOLH1 Antibody | Protein Microarray Validated

	Catalog No	Formulation	Size	Price (USD)
	V7505-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	100 ug	519
Formulation
	V7505-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide	20 ug	229
Formulation
	V7505SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	519
Formulation
	V7505IHC-7ML	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only	7 ml	519
Formulation

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IHC testing of FFPE human prostate cancer with FOLH1 antibody (clone FOLH1/2363). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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IHC testing of FFPE human prostate cancer with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE human prostate carcinoma with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human LNCaP cell lysate with FOLH1 antibody. Predicted molecular weight ~100 kDa.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using FOLH1 antibody. These results demonstrate the foremost specificity of the FOLH1/2363 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free FOLH1 antibody as confirmation of integrity and purity.

IHC testing of FFPE rat kidney tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE dog kidney tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC testing of FFPE cow brain tissue with FOLH1 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Availability	1-3 business days
Species Reactivity	Human, Rat, Dog, Cow
Format	Purified
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG2b, kappa
Clone Name	FOLH1/2363
Purity	Protein G affinity chromatography
UniProt	Q04609
Localization	Cytoplasmic, cell surface
Applications	Western blot : 0.5-2ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations	This FOLH1 antibody is available for research use only.
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Description

Folate hydrolase 1 (FOLH1), also known as Prostate-specific membrane antigen (PSMA), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. FOLH1 has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Application Notes

Optimal dilution of the FOLH1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.