Filaggrin Antibody / FLG [clone FLG/1563] (V3840)

IHC analysis of Filaggrin using Filaggrin antibody (clone FLG/1563). Formalin-fixed, paraffin-embedded human skin tissue was analyzed. Brown chromogenic signal is observed along the epidermal layer, consistent with Filaggrin-positive stratified squamous epithelial cells. Required heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes prior to staining.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Filaggrin antibody (clone FLG/1563). These results demonstrate the foremost specificity of the FLG/1563 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Filaggrin antibody (clone FLG/1563) as confirmation of integrity and purity.