FGF21 Antibody / Metabolic Signaling Hormone Antibody [clone FGF21/4343] (V9462)

FGF21 Antibody Microarray Specificity Validation. Analysis of a HuProt(TM) human protein microarray containing more than 19,000 full-length human proteins using clone FGF21/4343 demonstrates highly selective binding to Fibroblast growth factor 21 / FGF21 with minimal off-target reactivity detected across the tested human proteome. The strong enrichment of FGF21 signal relative to lower-ranking proteins supports the specificity of this monoclonal antibody for studies involving metabolic signaling, endocrine regulation, hepatokine biology, and adaptive energy homeostasis pathways.
Z- and S-score interpretation: The Z-score represents the signal intensity generated when an antibody, together with a fluorescently labeled secondary antibody, binds to a specific protein target on the HuProt(TM) array. Z-scores are expressed as standard deviations above the mean signal intensity of all proteins present on the array. Proteins are ranked according to descending Z-score values, and the S-score represents the difference in Z-score between sequentially ranked proteins. The S-score therefore reflects the relative specificity of an antibody for its intended target compared to other proteins represented on the array.

FGF21 Antibody Human Lung IHC. Immunohistochemistry analysis of FFPE human lung tissue stained with FGF21 Antibody / Metabolic Signaling Hormone Antibody clone FGF21/4343 demonstrates distinct cytoplasmic and membranous HRP-DAB brown staining within epithelial cell populations lining bronchial and alveolar-associated structures, consistent with the role of Fibroblast growth factor 21 / FGF21 in metabolic adaptation, endocrine signaling, and tissue-associated energy homeostasis pathways. The negative control inset using PBS in place of primary antibody demonstrates minimal background secondary antibody binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.