EWS Antibody / Ewing Sarcoma Protein Antibody (R36298)

EWS Antibody HeLa Nuclear WB. Western blot analysis of nuclear HeLa cell lysate using EWS Antibody at 0.3 ug/ml. A single prominent band is detected at approximately 90 kDa. Although the predicted molecular weight of EWS is approximately 69 kDa, the protein routinely migrates at a higher apparent molecular weight because of its intrinsically disordered domains and unusual amino acid composition, which alter electrophoretic mobility during SDS-PAGE. These results demonstrate that this EWS Antibody, an Ewing Sarcoma Protein Antibody, specifically detects endogenous nuclear EWS and is suitable for Western blot analysis.

EWS Antibody Human Kidney IHC. Immunohistochemical analysis of FFPE human kidney tissue following heat-induced epitope retrieval in pH6 citrate buffer. Tissue sections were incubated with EWS Antibody at 7 ug/ml, followed by an HRP-conjugated secondary antibody and chromogenic detection. Predominantly nuclear staining is observed throughout renal tubular epithelial cells, with minimal staining in glomeruli, consistent with the known nuclear localization of EWS as an RNA-binding protein involved in transcriptional regulation and RNA processing. This staining pattern demonstrates that this EWS Antibody, an Ewing Sarcoma Protein Antibody, is suitable for detecting endogenous EWS expression in human FFPE kidney tissue by immunohistochemistry.

EWS Antibody Human Kidney Negative Control IHC. Negative control immunohistochemical analysis of FFPE human kidney tissue following heat-induced epitope retrieval in pH6 citrate buffer. Tissue sections were processed using the same HRP staining protocol as the experimental sample but without the primary antibody. No specific DAB staining is observed, demonstrating minimal background signal and supporting the specificity of the EWS Antibody staining observed in the corresponding human kidney tissue section.