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Home >> Antibodies >> Estrogen Receptor Antibody (beta)

Estrogen Receptor Antibody (beta) (R30294)

  Catalog No Formulation Size Price (USD)  
Image R30294 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 329
Western blot testing of Estrogen receptor antibody and MCF-7 lysate (human breast cancer line). Predicted molecular weight: 53~59 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
UniProt Q92731
Applications Western blot : 0.5-1ug/ml
Limitations This Estrogen Receptor antibody is available for research use only.
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Estrogen receptor-beta, referred to as ESR2 or ERb, is a member of the superfamily of nuclear receptors which can transduce extracellular signals into transcriptional responses. This gene is mapped to 14q and comprises 8 exons spanning approximately 40 kb. It is expressed in multiple tissues, including developing spermatids of the testis and in ovarian granulosa cells. ERb is homologous to the previously identified ERa and has an overlapping but nonidentical tissue distribution. The DNA-binding domain of ERb is 96% conserved compared to ERa, and the ligand-binding domain shows 58% conserved residues. Estrogen receptor beta is expressed in human thymus, spleen, ovary, and testis. ERb mRNA was coexpressed with ERa and its splice variants in 60% of prolactinomas, 100% of mixed growth hormone /prolactin tumors, and 29% of gonadotroph tumors. Gene expression was not limited to ERa-positive tumor subtypes, however, and was also found in 100% of null cell tumors, 80% of somatotroph tumors, and 60% of corticotroph tumors.

Application Notes

The stated application concentrations are suggested starting amounts. Titration of the Estrogen Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.


An amino acid sequence from the N-terminus of human Estrogen Receptor beta (HGSIYIPSSYVDSHHEYPAMTFYS) was used as the immunogen for this Estrogen Receptor antibody.


After reconstitution, the Estrogen Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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