- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
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EPGN Antibody recognizes Epigen, an epidermal growth factor family protein also known as epithelial mitogen. Epigen is encoded by the EPGN gene and functions as a ligand for the epidermal growth factor receptor. It is synthesized as a membrane-associated precursor containing an extracellular EGF-like domain, a transmembrane region and a short intracellular segment. Proteolytic processing can release the extracellular ligand, allowing Epigen to signal to the producing cell or neighboring cells. Binding of Epigen to EGFR promotes receptor activation and can stimulate the growth of responsive epithelial cell populations.
Although Epigen interacts with EGFR with relatively low affinity, it can generate potent and sustained mitogenic signaling. Ligand binding promotes EGFR dimerization and receptor phosphorylation, followed by recruitment of intracellular signaling proteins. Downstream pathways may include the RAS-MAPK and PI3K-AKT cascades, which regulate cell-cycle progression, survival, differentiation and tissue growth. An EPGN Antibody can help researchers examine how Epigen abundance relates to EGFR activation and downstream signaling under different biological or experimental conditions.
Epigen expression is regulated according to tissue type, developmental stage and local signaling conditions. Studies of EPGN biology have associated the ligand with epithelial development, tissue remodeling, regenerative responses and the maintenance of selected proliferative cell populations. Its biological effects can be influenced by the availability of EGFR, proteolytic processing of the membrane precursor and the presence of other EGFR family ligands. Comparing Epigen with EGF, amphiregulin, epiregulin or transforming growth factor alpha may help reveal how individual ligands generate distinct receptor activation patterns and cellular responses.
EPGN Antibody products can support research into epithelial growth, receptor tyrosine kinase signaling and cancer-associated metabolic or proliferative pathways. This antibody is intended for detecting mouse and rat Epigen in validated applications, including western blot and immunohistochemistry. Analysis of brain, testis or other responsive tissues may help define tissue-specific EPGN protein distribution, while studies in disease models can assess changes associated with altered EGFR pathway activity. NSJ Bioreagents supplies this EPGN Antibody, also described as an EGFR Ligand Epigen Antibody, for investigating Epigen expression, epithelial mitogenic signaling and related growth-regulatory mechanisms.
For additional Epigen research products, including an antibody validated with human samples, visit our Epigen Antibody page covering epithelial mitogen expression and EGFR signaling.
Optimal dilution of the EPGN Antibody / EGFR Ligand Epigen Antibody should be determined by the researcher.
A recombinant mouse protein corresponding to amino acids A19-K109 was used as the immunogen for the Epigen / EPGN antibody.
After reconstitution, the Epigen / EPGN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Epigen Antibody, EGFR Ligand Epigen Antibody, Epithelial Mitogen Antibody, EPG Antibody, Proepigen Antibody, Epigen Precursor Antibody, EGF Family Ligand Epigen Antibody
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