EpCAM Antibody Cocktail [clone PAN-EpCAM] (V2694)

Description

This is a cocktail of four highly specific monoclonal antibodies (EGP40/826, EGP40/837, EGP40/1110, EGP40/1120) that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (Ep-CAM). It is a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or Ep-CAM. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

Application Notes

Optimal dilution of the EpCAM antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant human protein was used as the immunogen for the EpCAM antibody cocktail.

Storage

Store the EpCAM antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).