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Home >> Antibodies >> EIF4E Antibody

EIF4E Antibody (F47411)

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  Catalog No Formulation Size Price (USD)  
Image F47411-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 429
Image
F47411-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 199
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EIF4E antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a <a href=../search_result.php?search_txt=n1001>negative control</a> (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
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EIF4E antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human testis tissue stained with EIF4E antibody
Western blot analysis of EIF4E antibody and HL-60 lysate. Predicted molecular weight ~27 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P06730
Applications Western Blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This EIF4E antibody is available for research use only.
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Description

EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 27-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Application Notes

Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This EIF4E antibody was produced from rabbits immunized with a recombinant protein from human VGFR2.

Storage

Aliquot the EIF4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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