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Home >> Antibodies >> EIF4E Antibody

EIF4E Antibody (F47411)

  Catalog No Formulation Size Price (USD)  
Image F47411-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F47411-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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EIF4E antibody flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
IHC analysis of FFPE human testis tissue stained with EIF4E antibody
Western blot analysis of EIF4E antibody and HL-60 lysate. Predicted molecular weight ~27 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P06730
Applications Western Blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This EIF4E antibody is available for research use only.
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Description

EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 27-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Additional EIF4E antibody formats and validation data are available on our main EIF4E Antibody page.

Application Notes

Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

This EIF4E antibody was produced from rabbits immunized with a recombinant protein from human VGFR2.

Storage

Aliquot the EIF4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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