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Home >> Antibodies >> EIF4E Antibody

EIF4E Antibody [clone 8C8-C10-C12] (F54054)

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  Catalog No Formulation Size Price (USD)  
Image F54054-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7, 4) K562, 5) rat C6, 6) mouse NIH3T3, 7) rat PC12, 8) human HepG2, 9) hamster CHO-K1, 10) human Raji, 11) mouse Raw264.7 and 12) human Ramos cell lysates using EIF4E antibody at 1:2000. Predicted molecular weight ~27 kDa.
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Western blot testing of human 1) Jurkat, 2) HeLa, 3) MCF7, 4) K562, 5) rat C6, 6) mouse NIH3T3, 7) rat PC12, 8) human HepG2, 9) hamster CHO-K1, 10) human Raji, 11) mouse Raw264.7 and 12) human Ramos cell lysates using EIF4E antibody at 1:2000. Predicted molecular weight ~27 kDa.
Western blot testing of human 1) MDA-MB-468, 2) ZR75-1, 3) MCF7, 4) T47D and 5) hamster CHO-K1 cell lysates using EIF4E antibody at 1:1000. Predicted molecular weight ~27 kDa.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse lgG1
Clone Name 8C8-C10-C12
Purity Protein G affinity
UniProt P06730
Applications Western blot : 1:1000
Limitations This EIF4E antibody is available for research use only.
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Description

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the EIF4E antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A human recombinant protein was used as the immunogen for this EIF4E antibody.

Storage

Store the EIF4E antibody at -20oC.

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