ECD Antibody / SGT1 / Protein Ecdysoneless homolog (RQ6765)

Description

Protein Ecdysoneless homolog, known as ECD, is a conserved nuclear protein involved in cell cycle regulation, mRNA processing, and transcriptional control. ECD plays a critical role in the G1 to S phase transition and has been shown to interact with components of the retinoblastoma (Rb) pathway, making it an important factor in proliferation and oncogenesis. ECD has also been implicated in the stabilization and function of p53, further connecting it to cancer-related signaling networks.

Multiple isoforms of ECD exist due to alternative splicing events, which may influence the protein's functional specificity, subcellular localization, or interaction with molecular partners. These isoforms contribute to the complexity of ECD's biological roles across tissues and developmental stages. Understanding the differential expression and activity of these variants is crucial for deciphering ECD’s full spectrum of cellular functions.

A high-quality ECD antibody is essential for detecting ECD protein in various experimental applications. Researchers commonly use an ECD antibody in techniques such as western blot, immunohistochemistry, immunofluorescence, and ELISA to examine protein expression, localization, and interaction patterns. The availability of reliable ECD antibody reagents allows for the investigation of both canonical and isoform-specific forms of ECD, supporting studies in cell cycle dynamics, cancer biology, and gene regulation.

Application Notes

Optimal dilution of the ECD antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids L50-Q567) was used as the immunogen for the ECD antibody.

Storage

After reconstitution, the ECD antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.