DUSP1 Antibody (RQ6126)

DUSP1 Antibody Human Caco-2 FACS. Flow cytometric analysis of DUSP1 expression was performed using DUSP1 antibody in human Caco-2 cells. DUSP1, also known as Dual Specificity Phosphatase 1 or MKP1 (MAP Kinase Phosphatase 1), is a stress-inducible phosphatase that negatively regulates MAPK signaling through dephosphorylation of ERK, JNK, and p38 kinases. Cells were blocked with goat sera and stained with DUSP1 antibody at 1 ug/million cells. Red = cells alone, Green = isotype control, Blue = DUSP1 antibody. A distinct rightward shift of the DUSP1 antibody histogram relative to the isotype control demonstrates specific detection of DUSP1 expression in Caco-2 cells. These results support the utility of DUSP1 Antibody for studies of MAPK regulation, signal transduction, cellular stress responses, and epithelial cell biology.

DUSP1 Antibody Human Cell Line Panel WB. Western blot analysis of DUSP1 expression was performed using anti-DUSP1 antibody in human U-87 MG, HeLa, and A549 cell lysates. DUSP1, also known as Dual Specificity Phosphatase 1 or MKP1 (MAP Kinase Phosphatase 1), is a stress-inducible phosphatase that negatively regulates MAPK signaling by dephosphorylating ERK, JNK, and p38 kinases. Lane 1: U-87 MG lysate. Lane 2: HeLa lysate. Lane 3: A549 lysate. A specific immunoreactive band is detected at approximately 39 kDa in all three cell lines, corresponding to the predicted molecular weight of DUSP1. The widespread expression observed across glioblastoma, cervical carcinoma, and lung carcinoma cells is consistent with the fundamental role of DUSP1 in regulating cellular stress responses and MAPK-mediated signal transduction. These results support the utility of DUSP1 Antibody for studies of MAPK regulation, inflammation, cellular adaptation, and cancer biology.