DR3 Ligand Antibody / TNFRSF25 Ligand / VEGI [clone VEGI/13117] (V6048)

Immunohistochemistry of DR3 Ligand antibody in human placenta tissue. FFPE human placenta shows strong membranous and cytoplasmic HRP-DAB brown staining within trophoblastic epithelial cells lining chorionic villi, consistent with TNFRSF25 ligand / TNFSF15 expression in placental and endothelial-associated compartments. Clone VEGI/13117 was used as a monoclonal antibody for detection. An inset image shows PBS substituted for the primary antibody as a negative control, demonstrating absence of specific staining. Heat-induced epitope retrieval was performed by heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.

Immunohistochemistry of DR3 Ligand antibody in human testis tissue. FFPE human testis demonstrates cytoplasmic and membranous HRP-DAB brown staining within seminiferous tubule epithelial cells, including spermatogenic cells at varying stages of maturation, consistent with TNFRSF25 ligand / TNFSF15 expression in immune-regulatory and paracrine signaling contexts. Clone VEGI/13117 was used as a monoclonal antibody for detection. An inset image shows PBS substituted for the primary antibody as a negative control, confirming absence of specific staining. Heat-induced epitope retrieval was performed by heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95oC followed by cooling at room temperature for 20 minutes prior to staining.

SDS-PAGE Analysis of Purified DR3 Ligand/TNFRSF25 Ligand antibody (VEGI/13117). Confirmation of Purity and Integrity of Antibody.