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Home >> Antibodies >> DNA Polymerase lambda Antibody / POLL

DNA Polymerase lambda Antibody / POLL (RQ4359)

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  Catalog No Formulation Size Price (USD)  
Image RQ4359 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa, 2) COLO-320, 3) MCF7 and 4) HepG2 cell lysate with DNA Polymerase lambda antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa, also observed at ~68 kDa.
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Western blot testing of human 1) HeLa, 2) COLO-320, 3) MCF7 and 4) HepG2 cell lysate with DNA Polymerase lambda antibody at 0.5ug/ml. Predicted molecular weight ~63 kDa, also observed at ~68 kDa.
Flow cytometry testing of human U-2 OS cells with DNA Polymerase lambda antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= DNA Polymerase lambda antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt Q9UGP5
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/10^6 cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This DNA Polymerase lambda antibody is available for research use only.
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Description

DNA polymerase lambda, also known as POLL, is a protein found in eukaryotes. In humans, it is encoded by the POLLA gene. POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, suggesting that POLL participates in short- and long-patch base excision repair. POLL is widely expressed, with abundant expression in pachytene spermatocytes of testis and in ovary.

Application Notes

Optimal dilution of the DNA Polymerase lambda antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids K307-N407 was used as the immunogen for the DNA Polymerase lambda antibody.

Storage

After reconstitution, the DNA Polymerase lambda antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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