Cytokeratin 6 Antibody KRT6A/2368 / CK6 Monoclonal Antibody [clone KRT6A/2368] (V3850)

Cytokeratin 6 Antibody KRT6A/2368 by IHC in Human Basal Cell Carcinoma. Immunohistochemistry of FFPE human basal cell carcinoma tissue using Cytokeratin 6 Antibody KRT6A/2368. Strong brown cytoplasmic staining is observed throughout extensive epithelial tumor regions, with particularly prominent staining in densely packed tumor cell populations. The CK6 Monoclonal Antibody highlights the epithelial architecture, while adjacent connective tissue exhibits comparatively weaker staining. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed by boiling tissue sections in 10 mM citrate buffer, pH 6, for 10-20 minutes, followed by cooling at room temperature for 20 minutes.

Cytokeratin 6 Antibody KRT6A/2368 by IHC in Human Basal Cell Carcinoma, Second View. Immunohistochemistry of FFPE human basal cell carcinoma tissue using Cytokeratin 6 Antibody KRT6A/2368. Strong, relatively uniform brown cytoplasmic staining is observed across a broad sheet of tumor cells, with clearly visible nuclei and comparatively little staining in the adjacent connective tissue. The CK6 Monoclonal Antibody demonstrates extensive epithelial immunoreactivity, highlighting the distribution of cytokeratin-positive cells within the tumor. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes.

Cytokeratin 6 Antibody KRT6A/2368 by IHC in Human Prostate Carcinoma. Immunohistochemistry of FFPE human prostate carcinoma tissue using Cytokeratin 6 Antibody KRT6A/2368. Patchy brown staining is observed in epithelial cells associated with glandular structures, with variable intensity across the tissue section. Many epithelial cells exhibit relatively weak staining, while more prominent immunoreactivity is concentrated in selected glandular regions. The CK6 Monoclonal Antibody demonstrates heterogeneous cytokeratin immunoreactivity in this specimen. Nuclei are counterstained blue with hematoxylin. Heat-induced epitope retrieval was performed using 10 mM citrate buffer, pH 6, for 10-20 minutes.

Cytokeratin 6 Antibody KRT6A/2368 by Protein Microarray. Protein microarray analysis of Cytokeratin 6 Antibody KRT6A/2368 using a HuProt human protein microarray containing more than 19,000 full-length proteins. KRT6A produces the strongest binding signal, with a Z-score of 67.86 and S-score of 31.52. Secondary signals are detected for RCOR2 (Z-score 36.34; S-score 6.23) and KRT5 (Z-score 30.11; S-score 7.77). The CK6 Monoclonal Antibody demonstrates preferential recognition of KRT6A under the assay conditions, although the additional signals indicate potential secondary interactions that warrant consideration when evaluating target specificity.

Cytokeratin 6 Antibody KRT6A/2368 by SDS-PAGE. SDS-PAGE analysis of purified, BSA-free Cytokeratin 6 Antibody KRT6A/2368 under non-reducing (NR) and reducing (R) conditions, with 2 ug of antibody loaded per lane. A prominent band is observed at approximately 150 kDa under non-reducing conditions, consistent with intact mouse IgG. The reducing lane shows substantially weaker staining, without clearly resolved heavy and light chain bands at the expected molecular weights of approximately 50 kDa and 25 kDa. The CK6 Monoclonal Antibody demonstrates a prominent intact immunoglobulin band under non-reducing conditions.

Cytokeratin 6 Antibody KRT6A/2368 by FACS in Human HeLa Cells. Flow cytometry analysis of PFA-fixed human HeLa cells using Cytokeratin 6 Antibody KRT6A/2368. The blue histogram representing antibody staining shows a pronounced rightward fluorescence shift relative to the red isotype control histogram. The two populations exhibit clear separation, with the antibody-treated cells displaying substantially higher fluorescence intensity. The CK6 Monoclonal Antibody demonstrates intracellular antigen recognition in fixed HeLa cells, supporting its use for investigating cytokeratin expression by flow cytometry.