- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Cytokeratin 20 Antibody for WB / KRT20 Western Blot Antibody [clone KRT20/7805R] (V4493)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V4493-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
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V4493-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
|
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V4493SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | KRT20/7805R |
| Purity | Protein A/G affinity |
| UniProt | P35900 |
| Localization | Cytoplasm |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT Western Blot : 2-4ug/ml |
| Limitations | This Cytokeratin 20 antibody is available for research use only. |
|
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Keratin 20 (KRT20) is a type I acidic cytokeratin that belongs to the intermediate filament family of structural proteins responsible for maintaining epithelial cell integrity and cytoskeletal stability. Keratin 20 is predominantly localized in the cytoplasm of epithelial cells where it participates in intermediate filament networks that provide mechanical strength and structural organization. Cytokeratin 20 Antibody for WB (clone KRT20/7805R) enables detection of KRT20 protein in protein lysates by western blot, allowing researchers to analyze epithelial cytoskeletal protein expression and evaluate KRT20 levels across cell lines and tissue samples.
Keratin 20 is encoded by the KRT20 gene located on chromosome 17q21, a region containing several keratin family genes that are important for epithelial tissue structure. The protein is widely known in the literature as Cytokeratin 20 or CK20, two commonly used names in studies of epithelial biology and tumor marker research. Keratin 20 typically forms heterodimers with type II keratins such as keratin 8, and these heterodimers assemble into intermediate filament polymers that extend throughout the cytoplasm of epithelial cells. Because of its stable cytoskeletal association, KRT20 protein is commonly detected in western blot experiments as a characteristic band corresponding to the Keratin 20 polypeptide present in epithelial-derived samples.
Keratin 20 expression is strongly associated with differentiated epithelial tissues. High levels of KRT20 are observed in intestinal epithelial cells, gastric mucosa, and urothelial umbrella cells, where the protein contributes to epithelial barrier stability and cytoskeletal organization. Cytokeratin 20 antibody reagents are therefore widely used in research examining epithelial differentiation and epithelial lineage identity. Western blot detection using Cytokeratin 20 Antibody for WB allows quantitative comparison of KRT20 protein expression in epithelial cell lines, tumor samples, and experimental models studying epithelial development or differentiation.
In cancer biology, Keratin 20 is frequently retained in tumors derived from gastrointestinal and urothelial epithelia. CK20 expression is commonly observed in colorectal carcinoma, gastric carcinoma, pancreatic carcinoma, and bladder carcinoma, where it is used as a marker of epithelial differentiation. Western blot analysis with a Cytokeratin 20 Antibody for WB allows researchers to confirm KRT20 protein expression in tumor cell lysates and investigate changes in cytokeratin expression associated with epithelial transformation or tumor progression.
The recombinant rabbit monoclonal antibody clone KRT20/7805R targets Keratin 20 and can be used for research applications involving detection of KRT20 protein in cellular lysates and tissue extracts. In western blot experiments, Keratin 20 typically appears as a band corresponding to the full length intermediate filament protein, reflecting the presence of Cytokeratin 20 in epithelial derived samples. Detection of this protein supports studies of epithelial cytoskeletal composition, epithelial differentiation pathways, and epithelial tumor biology.
Optimal dilution of the Cytokeratin 20 Antibody for WB should be determined by the researcher.
A recombinant fragment of human KRT20 protein (within amino acids 324-424) was used as the immunogen for the Cytokeratin 20 antibody.
Aliquot the Cytokeratin 20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Keratin 20 antibody, CK20 antibody, KRT20 antibody, Cytokeratin-20 antibody, Keratin 20 protein antibody
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